Growth Factors & Cytokines

Recombinant Murine IL-4

Product Details

Catalogue Number: 214-14
Description:

IL-4 is a pleiotropic cytokine that regulates diverse T and B cell responses including cell proliferation, survival and gene expression. Produced by mast cells, T cells and bone marrow stromal cells, IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 cells, characterized by their cytokine-secretion profile that includes secretion of IL-4, IL-5, IL-6, IL-10, and IL-13, which favor a humoral immune response. Another dominant function of IL-4 is the regulation of immunoglobulin class switching to the IgG1 and IgE isotypes. Excessive IL-4 production by Th2 cells has been associated with elevated IgE production and allergy. Recombinant Murine IL-4 is a 13.5 kDa globular protein containing 121 amino acid residues.

Source: E.coli

Synonyms: BCGF, BCDF, B cell stimulating factor (BSF-1)

AA Sequence: MHIHGCDKNH LREIIGILNE VTGEGTPCTE MDVPNVLTAT KNTTESELVC RASKVLRIFY LKHGKTPCLK KNSSVLMELQ RLFRAFRCLD SSISCTMNES KSTSLKDFLE SLKSIMQMDY S

Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.

Biological Activity: The ED50 was determined by the dose-dependent proliferation of murine HT-2 cells is ≤ 2.0 ng/ml, corresponding to a specific activity of ≥ 5 x 105 units/mg.

This product has been tested against the WHO standard. International Unit information can be found here.

Calculated Molecular Weight: 13.5 kDa

Accession Number: P07750

Gene ID: 16189

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Vora, K A
Title
Severe attenuation of the B cell immune response in Msh2-deficient mice.
Citation
The Journal of Experimental Medicine; 189(3) pg471-82
PubMed Id
First Author
Kageyama, Y
Title
Reduced susceptibility to collagen-induced arthritis in mice deficient in IFN-gamma receptor.
Citation
Journal of immunology (Baltimore, Md. : 1950); 161(3) pg1542-8
PubMed Id
First Author
Hikida, M
Title
Expression of recombination activating genes in germinal center B cells: involvement of interleukin 7 (IL-7) and the IL-7 receptor.
Citation
The Journal of Experimental Medicine; 188(2) pg365-72
PubMed Id

product.subtitle.productreviews

Existing reviews
works well
Used to polarize RAW 264.7 macrophages.
From:  Akil | Date:  7/8/2020
Good
Fast shipping, good quantity.
From:  TONG | Date:  7/8/2020
Very Good
Used to M2 macrophage polarization and always works well.
From:  Yingjun | Date:  7/21/2020
Product with great consistency
We have been using this cytokine for immune cells extract from mouse spleen and bone marrow in numerous publications for the past 10 years. Great consistency!
From:  Jin | Date:  7/21/2020
Great consistent IL-4 cytokine for differentiation
We routinely use this cytokine to generate mouse CD4+ Th2 cells and for M2 macrophage polarization. The pricing and consistency of this cytokine is why we have stuck with peprotech for almost 20 years. Peprotech is our sole vendor for all cytokines.
From:  Chirag | Date:  8/31/2020