Anti-Human Galectin-3 0 ReviewsSubmit a Review Product Details Catalogue Number: 500-P246 Description: Source: Polyclonal Rabbit Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hGalectin-3. Anti-Human Galectin-3 specific antibody was purified by affinity chromatography employing immobilized hGalectin-3 matrix. Immunogen: E.coli derived Recombinant Human Galectin-3 (PeproTech catalog# 450-38) Sandwich ELISA: To detect hGalectin-3 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human Galectin-3 (500-P246Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hGalectin-3. Western Blot: To detect hGalectin-3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hGalectin-3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Note: Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information. crossreactivity: Human References SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest Apoptosis Cancer Immune System Inflammation product.subtitle.recentcitations First Author Kopcow, H D Title T cell apoptosis at the maternal-fetal interface in early human pregnancy, involvement of galectin-1. Citation Proceedings of the National Academy of Sciences of the United States of America; 105(47) pg18472-7 PubMed Id 19011096