Anticorps Polyclonaux Purifiés par Affinité à l’Antigène

Anti-Human GM-CSF

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Product Details

Catalogue Number: 500-P33

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hGM-CSF. Anti-Human GM-CSF specific antibody was purified by affinity chromatography employing immobilized hGM-CSF matrix.

Immunogen: E.coli derived Recombinant Human GM-CSF (PeproTech catalog# 300-03)

Sandwich ELISA: To detect hGM-CSF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human GM-CSF (500-P33Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hGM-CSF.

Anti-Human GM-CSF Sandwich ELISA

Western Blot: To detect hGM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hGM-CSF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Human GM-CSF Western Blot Reduced Anti-Human GM-CSF Western Blot Unreduced

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Chong, H C
Title
Regulation of epithelial-mesenchymal IL-1 signaling by PPARbeta/delta is essential for skin homeostasis and wound healing.
Citation
The Journal of Cell Biology; 184(6) pg817-31
PubMed Id
First Author
Bialek-Waldmann, J K
Title
Induced dendritic cells co-expressing GM-CSF/IFN-α/tWT1 priming T and B cells and automated manufacturing to boost GvL.
Citation
Molecular therapy. Methods & clinical development; 21 pg621-641
PubMed Id
First Author
Chiu, W K
Title
Generation and growth of CD28nullCD8+ memory T cells mediated by IL-15 and its induced cytokines.
Citation
Journal of immunology (Baltimore, Md. : 1950); 177(11) pg7802-10
PubMed Id