Anticorps Polyclonaux Purifiés par Affinité à l’Antigène

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hI-309. Anti-Human I-309 specific antibody was purified by affinity chromatography employing immobilized hI-309 matrix.

Immunogen: E.coli derived Recombinant Human I-309 (CCL1) (PeproTech catalog# 300-37)

Sandwich ELISA: To detect hI-309 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human I-309 (500-P110Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hI-309.

Western Blot: To detect human I-309 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant human I-309 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.