Anticorps Polyclonaux Purifiés par Affinité à l’Antigène

Anti-Human IL-1RA

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Product Details

Catalogue Number: 500-P209

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits immunized with highly pure recombinant Human IL-1RA. Anti-Human IL-1RA specific antibody was purified by affinity chromatography employing an immobilized Human IL-1RA matrix.

Immunogen: E.coli derived Recombinant Human IL-1RA (PeproTech catalog# 200-01RA)

Sandwich ELISA: To detect Human IL-1RA by sandwich ELISA (using 100μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human IL-1RA (500-P209Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IL-1RA.

Anti-Human IL-1RA Sandwich ELISA

Western Blot: To detect Human IL-1RA by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human IL-1RA is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Human IL-1RA Western Blot Reduced Anti-Human IL-1RA Western Blot Unreduced

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Bouhaddou, M
Title
The Global Phosphorylation Landscape of SARS-CoV-2 Infection.
Citation
Cell; 182(3) pg685-712.e19
PubMed Id
First Author
Blanco-Melo, D
Title
Imbalanced Host Response to SARS-CoV-2 Drives Development of COVID-19.
Citation
Cell; 181(5) pg1036-1045.e9
PubMed Id