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Anticorps Polyclonaux Purifiés par Affinité à l’Antigène

Anti-Human IL-3

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Product Details

Catalogue Number: 500-P23
Anti-Human IL-3

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-3. Anti-Human IL-3 specific antibody was purified by affinity chromatography employing immobilized hIL-3 matrix.

Immunogen: E.coli derived Recombinant Human IL-3 (PeproTech catalog# 200-03)

Sandwich ELISA: To detect hIL-3 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human IL-3 (500-P23Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-3.

Anti-Human IL-3 Sandwich ELISA

Western Blot: To detect hIL-3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Human IL-3 Western Blot Reduced Anti-Human IL-3 Western Blot Unreduced


Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

Country Of Origin: USA

Not for human use.

Research Interest