Anticorps Polyclonaux Purifiés par Affinité à l’Antigène

Anti-Human NT-3 (Polyclonal Rabbit)

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Product Details

Catalogue Number: 500-P82

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hNT-3. Anti-Human NT-3 specific antibody was purified by affinity chromatography employing immobilized hNT-3 matrix.

Immunogen: E.coli derived Recombinant Human NT-3 (PeproTech catalog# 450-03)

Sandwich ELISA: To detect hNT-3 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human NT-3 (500-P82Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hNT-3.

Western Blot: To detect hNT-3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNT-3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Miknyoczki, S J
Title
The neurotrophin-trk receptor axes are critical for the growth and progression of human prostatic carcinoma and pancreatic ductal adenocarcinoma xenografts in nude mice.
Citation
Clinical cancer research : an official journal of the American Association for Cancer Research; 8(6) pg1924-31
PubMed Id