Biotinylated Anti-Human MDC (CCL22) 0 ReviewsSubmit a Review Product Details Catalogue Number: 500-P107BT Description: Source: Polyclonal Rabbit Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hMDC. Anti-Human MDC specific antibody was purified by affinity chromatography and then biotinylated. Immunogen: E.coli derived Recombinant Human MDC (CCL22) (69 a.a.) (PeproTech catalog# 300-36A) Sandwich ELISA: To detect hMDC by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti-Human MDC (500-P107) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hMDC. Western Blot: To detect hMDC by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMDC is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Note: 1mg will be provided as 2x500μg. crossreactivity: Human References SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest AIDS/HIV Chemotaxis Immune System Wound Healing product.subtitle.recentcitations First Author Mateo, T Title Angiotensin II-induced mononuclear leukocyte interactions with arteriolar and venular endothelium are mediated by the release of different CC chemokines. Citation Journal of immunology (Baltimore, Md. : 1950); 176(9) pg5577-86 PubMed Id 16622027
