Anticorps Polyclonaux Biotinylés

Source: Polyclonal Goat

Preparation: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hFasL/Apo1L. Anti-Human FasL/Apo1L specific antibody was purified by affinity chromatography and then biotinylated.

Immunogen: CHO cells derived Recombinant Human sFas Ligand (PeproTech catalog# 310-03)*
*Catalog# 310-03 has been replaced with 310-03H.

Sandwich ELISA: To detect hFasL/Apo1L by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti-Human FasL/Apo1L (500-P184G) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hFasL/Apo1L.

Western Blot: To detect hFasL/Apo1L by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 mg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFasL/Apo1L is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Note:

1mg will be provided as 2x500μg.