Recombinant Human SPARC/Osteonectin 0 ReviewsSubmit a Review Product Details Catalogue Number: 120-36 Description: SPARC/Osteonectin is a secreted, evolutionarily-conserved, collagen-binding glycoprotein that is involved in a variety of cellular activities. It is highly expressed in tissues undergoing morphogenesis, remodeling and wound repair. SPARC/Osteonectin and its related peptides bind to numerous proteins of the extracellular matrix (ECM), affect ECM protein expression, influence cellular adhesion and migration, and modulate growth factor-induced cell proliferation and angiogenesis. SPARC/Osteonectin consists of three domains: an N-terminal acidic region that binds calcium ions with low affinity, a module containing two EF-hand motifs that bind calcium with high affinity, and a cysteine-rich follistatin-like domain. Recombinant Human SPARC/Osteonectin is a glycoprotein containing 286 amino acids that migrates at an apparent MW of 43.7 kDa by SDS-PAGE analysis due to the effect of glycosylation. The calculated molecular weight of Recombinant Human SPARC/Osteonectin is 32.7 kDa. Source: CHO cells Synonyms: Secreted protein acidic and rich in cysteine, BM-40, ON AA Sequence: APQQEALPDE TEVVEETVAE VTEVSVGANP VQVEVGEFDD GAEETEEEVV AENPCQNHHC KHGKVCELDE NNTPMCVCQD PTSCPAPIGE FEKVCSNDNK TFDSSCHFFA TKCTLEGTKK GHKLHLDYIG PCKYIPPCLD SELTEFPLRM RDWLKNVLVT LYERDEDNNL LTEKQKLRVK KIHENEKRLE AGDHPVELLA RDFEKNYNMY IFPVHWQFGQ LDQHPIDGYL SHTELAPLRA PLIPMEHCTT RFFETCDLDN DKYIALDEWA GCFGIKQKDI DKDLVI Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses. Biological Activity: Determined by its ability to inhibit alkaline phosphatase activity in differentiating MC3T3 cells, with an IC50 of 0.5-0.7 μg/ml. Calculated Molecular Weight: 32.7 kDa Accession Number: P09486 Gene ID: 6678 Endotoxin: Endotoxin level is < 0.1 ng/ug of protein (< 1 EU/ug) crossreactivity: Human, Mouse, Rat References PubMed SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest Angiogenesis/Cardiovascular Bone, Skeletal, Cartilage Cancer Immune System Wound Healing product.subtitle.recentcitations First Author Yao, L Title Bidirectional epithelial-mesenchymal crosstalk provides self-sustaining profibrotic signals in pulmonary fibrosis. Citation The Journal of Biological Chemistry; 297(3) pg101096 PubMed Id 34418430 First Author Zhu, J Title SPARC promotes self-renewal of limbal epithelial stem cells and ocular surface restoration through JNK and p38-MAPK signaling pathways. Citation Stem Cells; 38(1) pg134-145 PubMed Id 31644832 First Author John, B Title Regulation of the bi-directional cross-talk between ovarian cancer cells and adipocytes by SPARC. Citation Oncogene; 38(22) pg4366-4383 PubMed Id 30765860
