Animal-Free Cytokines

Animal-Free Recombinant Human IL-6

Product Details

Catalog Number: AF-200-06
Description:

IL-6 is a pleiotropic cytokine that plays an important role in host defense by regulating immune and inflammatory responses. Produced by T cells, monocytes, fibroblasts, endothelial cells and keratinocytes, IL-6 has diverse biological functions. It stimulates B cell differentiation and antibody production, synergizes with IL-3 in megakaryocyte development and platelet production, induces expression of hepatic acute-phase proteins, and regulates bone metabolism. IL-6 signals through the IL-6 receptor system that consists of two chains, IL-6Rα and gp130. Murine IL-6 is inactive on human cells, while both human and murine are equally active on murine cells. Recombinant Human IL-6 is a 20.9 kDa protein containing 184 amino acid residues.

Manufactured using all Animal-Free reagents.

Source: E.coli

Synonyms: 26 kDa protein, IFN-β2, B cell differentiation factor (BCDF), BSF-2, HPGF, HSF, MGI-2

AA Sequence: PVPPGEDSKD VAAPHRQPLT SSERIDKQIR YILDGISALR KETCNKSNMC ESSKEALAEN NLNLPKMAEK DGCFQSGFNE ETCLVKIITG LLEFEVYLEY LQNRFESSEE QARAVQMSTK VLIQFLQKKA KNLDAITTPD PTTNASLLTK LQAQNQWLQD MTTHLILRSF KEFLQSSLRA LRQM

Purity: Greater than 98% by SDS-PAGE gel and HPLC analyses.

Biological Activity: The ED50 was determined by the dose-dependent stimulation of the proliferation of the IL-6 dependent murine 7TD1 cells is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg.

Cross Reactivity:
Human, Monkey, Mouse, Rat
Research Interest

Country Of Origin: USA

Research Use Only: Not for use in diagnostic or therapeutic procedures.