Produced in BALB/c ICRR F1 mice using highly pure (>98%) recombinant human Eotaxin-3 as the immunizing antigen. This IgG2aK antibody was purified from ascites fluid by Protein A affinity chromatography.
E.coli derived Recombinant Human Eotaxin-3 (CCL26) (PeproTech catalog# 300-48)
In a sandwich ELISA (assuming 100μl/well), a concentration of 1.0-2.0 μg/ml of this antibody will detect at least 1000pg/ml of Recombinant Human Eotaxin-3 when used with PeproTech’s Biotinylated Antigen Affinity Purified Anti-Human Eotaxin-3 (CCL26) (500-P156GBT) as the detection antibody at a concentration of approximately 0.25-0.50 μg/ml.
To detect Human Eotaxin-3 by Western Blot analysis this antibody can be used at a concentration of 0.25- 0.50 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human Eotaxin-3 is 0.25-0.5 ng/lane, under reducing or non-reducing conditions.
Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.