Anti-Human IFN-γ 0 ReviewsSubmit a Review Product Details Catalog Number: 500-P32 Description: Source: Polyclonal Rabbit Preparation: Produced from sera of rabbits pre-immunized with highly pure recombinant Human IFN-γ. Anti-Human IFN-γ specific antibody was purified by affinity chromatography employing immobilized Human IFN-γ matrix. Immunogen: E.coli derived Recombinant Human IFN-γ (PeproTech catalog# 300-02) Sandwich ELISA: To detect Human IFN-γ by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human IFN-γ (500-P32Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IFN-γ. Western Blot: To detect Human IFN-γ by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IFN-γ is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. Note: Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information. Cross Reactivity Cited in References: Bacteria, Human References SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest COVID-19 AIDS/HIV Angiogenesis/Cardiovascular Cancer Diabetes/Weight Regulation Immune System Neurobiology Stem Cells & Differentiation Allergy Transplantation Recent Citations First Author Sanderson, N S Title Cytotoxic immunological synapses do not restrict the action of interferon-γ to antigenic target cells. Citation Proceedings of the National Academy of Sciences of the United States of America; 109(20) pg7835-40 PubMed ID 22547816 First Author Bradshaw, E M Title Concurrent detection of secreted products from human lymphocytes by microengraving: cytokines and antigen-reactive antibodies. Citation Clinical immunology (Orlando, Fla.); 129(1) pg10-8 PubMed ID 18675591 First Author Takagi, R Title B cell chemoattractant CXCL13 is preferentially expressed by human Th17 cell clones. Citation Journal of immunology (Baltimore, Md. : 1950); 181(1) pg186-9 PubMed ID 18566383