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Recombinant Human sCD14

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Product Details

Catalogue Number: 110-01
Description:

CD14 is a cell surface-anchored glycoprotein that is expressed predominantly by monocytes and tissue macrophages. CD14 associates with MD-2 (LY-96) and TLR4 to form a receptor complex, which signals specifically in response to bacterial lipopolysaccharide (LPS) binding. The CD14/MD-2/TLR4 receptor complex signals via MyD88, TIRAP, and TRAF6, and ultimately activates NF-κB. CD14 also exists in a soluble form, designated as sCD14, which is capable of specifically binding LPS in the extracellular space. Recombinant Human sCD14 is a 331 amino acid glycoprotein comprising the extracellular portion of the CD14 receptor.  The calculated molecular weight of Recombinant Human sCD14 is 35.6 kDa.

Source: HEK293 cells

Synonyms: soluble CD14, Monocyte differentiation antigen CD14

AA Sequence: TTPEPCELDD EDFRCVCNFS EPQPDWSEAF QCVSAVEVEI HAGGLNLEPF LKRVDADADP RQYADTVKAL RVRRLTVGAA QVPAQLLVGA LRVLAYSRLK ELTLEDLKIT GTMPPLPLEA TGLALSSLRL RNVSWATGRS WLAELQQWLK PGLKVLSIAQ AHSPAFSCEQ VRAFPALTSL DLSDNPGLGE RGLMAALCPH KFPAIQNLAL RNTGMETPTG VCAALAAAGV QPHSLDLSHN SLRATVNPSA PRCMWSSALN SLNLSFAGLE QVPKGLPAKL RVLDLSCNRL NRAPQPDELP EVDNLTLDGN PFLVPGTALP HEGSMNSGVV P

Purity: ≥ 95% by SDS-PAGE gel and HPLC analyses.

Biological Activity: Determined by the dose-dependent activation of NF-κB in a RAW264 cell line based reporter system, using a sCD14 concentration range of 20 ng/μl to 200 ng/μl. The NF-κB activation is enhanced when the assay is done in the presence of 0.25 ng/μl to 1.0 ng/μl bacterial LPS.

Calculated Molecular Weight: 35.6 kDa

Accession Number: P08571

Gene ID: 929

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Andrukhov, O
Title
Response of human periodontal ligament stem cells to IFN-γ and TLR-agonists.
Citation
Scientific Reports; 7(1) pg12856
PubMed Id
First Author
Ishida, A
Title
Negative regulation of Toll-like receptor-4 signaling through the binding of glycosylphosphatidylinositol-anchored glycoprotein, CD14, with the sialic acid-binding lectin, CD33.
Citation
The Journal of Biological Chemistry; 289(36) pg25341-50
PubMed Id
First Author
Ranoa, D R
Title
Human lipopolysaccharide-binding protein (LBP) and CD14 independently deliver triacylated lipoproteins to Toll-like receptor 1 (TLR1) and TLR2 and enhance formation of the ternary signaling complex.
Citation
The Journal of Biological Chemistry; 288(14) pg9729-41
PubMed Id