Produced in BALB/c mice immunized with highly pure Recombinant Human MCP-1 (CCL2). Anti-Human MCP-1 (CCL2)-specific antibody was purified from cell culture supernatant by Protein A affinity chromatography.
E.coli derived Recombinant Human MCP-1 (CCL2) (PeproTech catalog# 300-04)
In a sandwich ELISA (assuming 100μl/well), a concentration of 1.0-2.0 μg/ml of this antibody will detect at least 100pg/ml of Recombinant Human MCP-1 when used with PeproTech’s Biotinylated Antigen Affinity Purified anti-Human MCP-1 (CCL2) (500-P34BT) as the detection antibody at a concentration of approximately 0.5-1.0 μg/ml.
To detect Human MCP-1 by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human MCP-1 is 1.0-2.0 ng/lane under reducing conditions and 0.25-0.50 ng/lane under non-reducing conditions.
Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.