Antigen Affinity Purified Polyclonal Antibodies

Anti-Human SDF-1α (CXCL12)

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Product Details

Catalogue Number: 500-P87A
Description:
Anti-Human SDF-1α (CXCL12)
 

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human SDF-1alpha (CXCL12). Anti-Human SDF-1alpha (CXCL12)-specific antibody was purified by affinity chromatography employing immobilized Human SDF-1alpha (CXCL12) matrix.

Immunogen: E.coli derived Recombinant Human SDF-1α (CXCL12) (PeproTech catalog# 300-28A)

Sandwich ELISA: To detect Human SDF-1α by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human SDF-1α (500-P87ABt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human SDF-1α.

Anti-Human SDF-1α (CXCL12) Sandwich ELISA

Western Blot: To detect Human SDF-1α by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human SDF-1α is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Human SDF-1α (CXCL12) Western Blot Reduced Anti-Human SDF-1α (CXCL12) Western Blot Unreduced

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Hinderer, S
Title
Surface functionalization of electrospun scaffolds using recombinant human decorin attracts circulating endothelial progenitor cells.
Citation
Scientific Reports; 8(1) pg110
PubMed Id
First Author
Rao, S
Title
CXCL12 mediates trophic interactions between endothelial and tumor cells in glioblastoma.
Citation
PLoS ONE; 7(3) pge33005
PubMed Id
First Author
Cruz-Orengo, L
Title
CXCR7 influences leukocyte entry into the CNS parenchyma by controlling abluminal CXCL12 abundance during autoimmunity.
Citation
The Journal of Experimental Medicine; 208(2) pg327-39
PubMed Id