Anti-Rat IL-4 0 ReviewsSubmit a Review Product Details Catalog Number: 500-P94 Description: Source: Polyclonal Rabbit Preparation: Produced from sera of rabbits pre-immunized with highly pure recombinant Rat IL-4. Anti-Rat IL-4 specific antibody was purified by affinity chromatography employing immobilized Rat IL-4 matrix. Immunogen: E.coli derived Recombinant Rat IL-4 (PeproTech catalog# 400-04) Sandwich ELISA: To detect Rat IL-4 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Rat IL-4 (500-P94Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-4. Western Blot: To detect Rat IL-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Rat IL-4 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. Note: Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information. Cross Reactivity Cited in References: Rat References SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest COVID-19 Apoptosis Cancer Cell Culture Immune System Inflammation Proliferation Stem Cells & Differentiation Allergy Transplantation Recent Citations First Author Mateo, T Title A critical role for TNFalpha in the selective attachment of mononuclear leukocytes to angiotensin-II-stimulated arterioles. Citation Blood; 110(6) pg1895-902 PubMed ID 17595330 First Author Schif-Zuck, S Title Coadministration of plasmid DNA constructs encoding an encephalitogenic determinant and IL-10 elicits regulatory T cell-mediated protective immunity in the central nervous system. Citation Journal of immunology (Baltimore, Md. : 1950); 177(11) pg8241-7 PubMed ID 17114502 First Author Wildbaum, G Title Tr1 cell-dependent active tolerance blunts the pathogenic effects of determinant spreading. Citation The Journal of Clinical Investigation; 110(5) pg701-10 PubMed ID 12208871