Animal-Free Cytokines

Animal-Free Recombinant Human FGF-basic (154 a.a.)

Product Details

Catalogue Number: AF-100-18B
Description:

FGF-basic is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development, postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-basic is a non-glycosylated, heparin-binding growth factor that is expressed in the brain, pituitary, kidney, retina, bone, testis, adrenal gland, liver, monocytes, epithelial cells and endothelial cells. FGF-basic signals through FGFR 1b, 1c, 2c, 3c and 4. Recombinant Human FGF-basic is a 17.2 kDa protein consisting of 154 amino acid residues.

Qualified for stem cell use.

Manufactured using all Animal-Free reagents.

Source: E.coli

Synonyms: Fibroblast Growth Factor-basic, FGF-2, HBGF-2, Prostatropin

AA Sequence: AAGSITTLPA LPEDGGSGAF PPGHFKDPKR LYCKNGGFFL RIHPDGRVDG VREKSDPHIK LQLQAEERGV VSIKGVCANR YLAMKEDGRL LASKCVTDEC FFFERLESNN YNTYRSRKYT SWYVALKRTG QYKLGSKTGP GQKAILFLPM SAKS

Purity: ≥ 95% by SDS-PAGE gel and HPLC analyses.

Biological Activity: Determined by a cell proliferation assay using Balb/c 3T3 cells. The expected ED50 is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg.

This product has been tested against the WHO standard. International Unit information can be found here.

Calculated Molecular Weight: 17.2 kDa

Accession Number: P09038

Gene ID: 2247

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Xu, L
Title
EZH2 facilitates BMI1-dependent hepatocarcinogenesis through epigenetically silencing microRNA-200c
Citation
Oncogenesis; 9(11) pg101
PubMed Id
First Author
Zhu, Y
Title
LncRNA NEAT1 remodels chromatin to promote the 5-Fu resistance by maintaining colorectal cancer stemness
Citation
Cell Death & Disease; 11(11) pg962
PubMed Id
First Author
Hiemer, S E
Title
The transcriptional regulators TAZ and YAP direct transforming growth factor β-induced tumorigenic phenotypes in breast cancer cells.
Citation
The Journal of Biological Chemistry; 289(19) pg13461-74
PubMed Id

product.subtitle.productreviews

Existing reviews
Worked well for research purposes
We used this product in one of our orthopedic research studies. Product arrived quickly and was packaged well. Would purchase again.
From:  Emilie | Date:  8/26/2020