Anticorps Polyclonaux Purifiés par Affinité à l’Antigène

Anti-Human sTRAIL/Apo2L

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Product Details

Catalogue Number: 500-P135

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human sTRAIL/Apo2L. Anti-Human sTRAIL/Apo2L-specific antibody was purified by affinity chromatography employing immobilized Human sTRAIL/Apo2L matrix.

Immunogen: E.coli derived Recombinant Human sTRAIL/Apo2L (PeproTech catalog# 310-04)

Sandwich ELISA: To detect hsTRAIL/Apo2L by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human sTRAIL/Apo2L (500-P135Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hsTRAIL/Apo2L.

Anti-Human sTRAIL/Apo2L Sandwich ELISA

Western Blot: To detect hsTRAIL/Apo2L by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsTRAIL/Apo2L is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Human sTRAIL/Apo2L Western Blot Reduced Anti-Human sTRAIL/Apo2L Western Blot Unreduced


Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

Country Of Origin: USA

Not for human use.

Research Interest


First Author
Tatzel, K
Membrane-proximal TRAIL species are incapable of inducing short circuit apoptosis signaling: Implications for drug development and basic cytokine biology.
Scientific Reports; 6 pg22661
PubMed Id
First Author
Kemp, T J
Neutrophil stimulation with Mycobacterium bovis bacillus Calmette-Guerin (BCG) results in the release of functional soluble TRAIL/Apo-2L.
Blood; 106(10) pg3474-82
PubMed Id
First Author
Ludwig, A T
Tumor necrosis factor-related apoptosis-inducing ligand: a novel mechanism for Bacillus Calmette-Guérin-induced antitumor activity.
Cancer Research; 64(10) pg3386-90
PubMed Id