Anticorps Polyclonaux Biotinylés

Biotinylated Anti-Human IFN-β

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Product Details

Catalogue Number: 500-P32BBT

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure recombinant Human IFN-β. Anti-Human IFN-β specific antibody was purified by affinity chromatography and then biotinylated.

Immunogen: E.coli derived Recombinant Human IFN-β (PeproTech catalog# 300-02B)*
*Catalog# 300-02B has been replaced with 300-02BC.

Sandwich ELISA: To detect Human IFN-β by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti-Human IFN-β (500-P32B) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Human IFN-β.

Biotinylated Anti-Human IFN-β Sandwich ELISA

Western Blot: To detect Human IFN-β by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IFN-β is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Biotinylated Anti-Human IFN-β Western Blot Unreduced Biotinylated Anti-Human IFN-β Western Blot Reduced

Note:

1mg will be provided as 2x500μg.

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Siednienko, J
Title
Nuclear factor κB subunits RelB and cRel negatively regulate Toll-like receptor 3-mediated β-interferon production via induction of transcriptional repressor protein YY1.
Citation
The Journal of Biological Chemistry; 286(52) pg44750-63
PubMed Id
First Author
Kölle, J
Title
Respiratory infections regulated blood cells IFN-β-PD-L1 pathway in pediatric asthma.
Citation
Immunity, Inflammation and Disease; 8(3) pg310-319
PubMed Id