Recombinant Human IL-13 Variant 0 ReviewsSubmit a Review Product Details Catalogue Number: 200-13A Description: IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells, and also by mast cells and NK cells. Targeted deletion of IL-13 in mice resulted in impaired Th2 cell development and indicated an important role for IL-13 in the expulsion of gastrointestinal parasites. IL-13 exerts anti-inflammatory effects on monocytes and macrophages and it inhibits the expression of inflammatory cytokines such as IL-1β, TNF-α, IL-6 and IL-8. IL-13 has also been shown to enhance B cell proliferation and to induce isotype switching, resulting in increased production of IgE. Blocking of IL-13 activity inhibits the pathophysiology of asthma. Human and murine IL-13 are cross-species reactive. A variant of IL-13 shows enhanced functional activity compared with the wild type IL-13. PeproTech’s genetic variant, termed Recombinant Human IL-13 Variant, is a mature 115 amino acid protein with a substitution of Q for R at position 113. The calculated molecular weight of Recombinant Human IL-13 Variant is 12.6 kDa. Source: E.coli Synonyms: None AA Sequence: MSPGPVPPST ALRELIEELV NITQNQKAPL CNGSMVWSIN LTAGMYCAAL ESLINVSGCS AIEKTQRMLS GFCPHKVSAG QFSSLHVRDT KIEVAQFVKD LLLHLKKLFR EGQFN Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses. Biological Activity: This IL-13 analog shows a two fold increase, relative to wild type IL-13, in bioactivity as measured by the in-vitro dose dependent activation of STAT6 and IL-13 dependent gene induction in transfected A201.1 cells. This analog has also been shown to exhibit increased in vivo activity compared to wild type IL-13, as measured by the induction of airway hyper-responsiveness. Calculated Molecular Weight: 12.6 kDa Accession Number: P35225 Gene ID: 3596 Endotoxin: Endotoxin level is < 0.1 ng/ug of protein (< 1 EU/ug) crossreactivity: Human, Mouse References PubMed SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest AIDS/HIV Cancer Immune System Inflammation Stem Cells & Differentiation Allergy product.subtitle.recentcitations First Author Miller, R Title Development of an in vitro potency bioassay for therapeutic IL-13 antagonists: the A-549 cell bioassay. Citation Journal of Immunological Methods; 334(1-2) pg134-41 PubMed Id 18355834 First Author Chen, W Title Functional effect of the R110Q IL13 genetic variant alone and in combination with IL4RA genetic variants. Citation The Journal of Allergy and Clinical Immunology; 114(3) pg553-60 PubMed Id 15356556