Semaphorins are a large group of structurally-related, secreted, GPI-anchored, transmembrane, cell-signaling molecules. There are 8 major classifications of Semaphorins (the first seven ordered by number, 1-7, and the eighth designated V for virus), which are characterized by the existence of a conserved 500 amino acid SEMA domain at the amino terminus. Classes 3, 4, 6, and 7 are found in vertebrates only, whilst class 5 is found in both vertebrates and invertebrates. Each class is then divided into additional subgroups based on shared structural characteristics. Semaphorins primarily function as axon growth cone guidance factors during neuronal development. Semaphorin 3A acts as a chemo-repellent to axons, and an inhibitor of the growth of axons by signaling through receptors, Neuropilin-1 and Plexin-A. PeproTech's CHO cell-derived Recombinant Human Semaphorin 3A Fc is a glycosylated, disulfide-linked homodimer of 1,976 amino acid residues, which includes the SEMA domain, immunoglobulin c2-like domain, and the C-terminal basic Arg/Lys-rich domain of the mature sequence, as well as an 8-residue N-terminal His-tag and a 230-residue C-terminal Fc region linked by two glycines. Recombinant Human Semaphorin 3A Fc has a calculated molecular weight of 226.2 kDa and therefore runs above the 200kDa marker by SDS-PAGE analysis under nonreducing conditions. When run under reducing conditions, this protein migrates as three distinct bands that, due to glycosylation, run higher than expected at apparent molecular weights of approximately 120-130 kDa, 90-100 kDa, and 35-40 kDa.
AA Sequence (monomer):
HHHHHHHHGK NNVPRLKLSY KEMLESNNVI TFNGLANSSS YHTFLLDEER SRLYVGAKDH IFSFDLVNIK DFQKIVWPVS YTRRDECKWA GKDILKECAN FIKVLKAYNQ THLYACGTGA FHPICTYIEI GHHPEDNIFK LENSHFENGR GKSPYDPKLL TASLLIDGEL YSGTAADFMG RDFAIFRTLG HHHPIRTEQH DSRWLNDPKF ISAHLISESD NPEDDKVYFF FRENAIDGEH SGKATHARIG QICKNDFGGH RSLVNKWTTF LKARLICSVP GPNGIDTHFD ELQDVFLMNF KDPKNPVVYG VFTTSSNIFK GSAVCMYSMS DVRRVFLGPY AHRDGPNYQW VPYQGRVPYP RPGTCPSKTF GGFDSTKDLP DDVITFARSH PAMYNPVFPM NNRPIVIKTD VNYQFTQIVV DRVDAEDGQY DVMFIGTDVG TVLKVVSIPK ETWYDLEEVL LEEMTVFREP TAISAMELST KQQQLYIGST AGVAQLPLHR CDIYGKACAE CCLARDPYCA WDGSACSRYF PTAKRATRAQ DIRNGDPLTH CSDLHHDNHH GHSPEERIIY GVENSSTFLE CSPKSQRALV YWQFQRRNEE RKEEIRVDDH IIRTDQGLLL RSLQQKDSGN YLCHAVEHGF IQTLLKVTLE VIDTEHLEEL LHKDDDGDGS KTKEMSNSMT PSQKVWYRDF MQLINHPNLN TMDEFCEQVW KRDRKQRRQR PGHTPGNSNK WKHLQENKKG RNRRTHEFER APRSVGGPKS CDKTHTCPPC PAPELLGGPS VFLFPPKPKD TLMISRTPEV TCVVVDVSHE DPEVKFNWYV DGVEVHNAKT KPREEQYNST YRVVSVLTVL HQDWLNGKEY KCKVSNKALP APIEKTISKA KGQPREPQVY TLPPSRDELT KNQVSLTCLV KGFYPSDIAV EWESNGQPEN NYKTTPPVLD SDGSFFLYSK LTVDKSRWQQ GNVFSCSVMH EALHNHYTQK SLSLSPGK
≥ 95% by SDS-PAGE gel and HPLC analyses.
Determined by its ability to bind recombinant rat Neuropilin‐1 Fc Chimera in a functional ELISA assay.
100μg is the largest vial size for this product.
Larger sizes supplied as multiple 100μg vials.
Calculated Molecular Weight:
Endotoxin level is < 0.1 ng/ug of protein (< 1 EU/ug)
Not for human use.