Antigen Affinity Purified Polyclonal Antibodies

Anti-Human IL-1α

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Product Details

Catalogue Number: 500-P21A

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-1α. Anti-Human IL-1α specific antibody was purified by affinity chromatography employing immobilized hIL-1α matrix.

Immunogen: E.coli derived Recombinant Human IL-1α (PeproTech catalog# 200-01A)

Sandwich ELISA: To detect hIL-1α by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human IL-1α (500-P21ABt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-1α.

Anti-Human IL-1α Sandwich ELISA Anti-Human IL-1α Sandwich ELISA

Western Blot: To detect hIL-1α by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-1α is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Human IL-1α Western Blot Reduced Anti-Human IL-1α Western Blot Unreduced Anti-Human IL-1α Western Blot Reduced Anti-Human IL-1α Western Blot Unreduced

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Burzynski, L C
Title
Interleukin-1α Activity in Necrotic Endothelial Cells Is Controlled by Caspase-1 Cleavage of Interleukin-1 Receptor-2: IMPLICATIONS FOR ALLOGRAFT REJECTION.
Citation
The Journal of Biological Chemistry; 290(41) pg25188-96
PubMed Id
First Author
Tsuchiya, K
Title
Gasdermin D mediates the maturation and release of IL-1α downstream of inflammasomes.
Citation
Cell Reports; 34(12) pg108887
PubMed Id
First Author
Chong, H C
Title
Regulation of epithelial-mesenchymal IL-1 signaling by PPARbeta/delta is essential for skin homeostasis and wound healing.
Citation
The Journal of Cell Biology; 184(6) pg817-31
PubMed Id