Antigen Affinity Purified Polyclonal Antibodies

Anti-Human IL-21

Product Details

Catalogue Number: 500-P191

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-21. Anti-Human IL-21 specific antibody was purified by affinity chromatography employing immobilized hIL-21 matrix.

Immunogen: E.coli derived Recombinant Human IL-21 (PeproTech catalog# 200-21)

Sandwich ELISA: To detect hIL-21 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human IL-21 (500-P191Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-21.

Anti-Human IL-21 Sandwich ELISA

Western Blot: To detect hIL-21 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-21 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Human IL-21 Western Blot Reduced Anti-Human IL-21 Western Blot Unreduced

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Nagy, N
Title
Soluble factors produced by activated CD4+ T cells modulate EBV latency.
Citation
Proceedings of the National Academy of Sciences of the United States of America; 109(5) pg1512-7
PubMed Id
First Author
Lamprecht, B
Title
Aberrant expression of the Th2 cytokine IL-21 in Hodgkin lymphoma cells regulates STAT3 signaling and attracts Treg cells via regulation of MIP-3alpha.
Citation
Blood; 112(8) pg3339-47
PubMed Id
First Author
Ma, C S
Title
Early commitment of naïve human CD4(+) T cells to the T follicular helper (T(FH)) cell lineage is induced by IL-12.
Citation
Immunology and Cell Biology; 87(8) pg590-600
PubMed Id

product.subtitle.productreviews

Existing reviews
great product
cleanest form of IL-21 we have used
From:  Thomas | Date:  26/08/2020