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Biotinylated Polyclonal Antibodies

Biotinylated Anti-Human IL-12 (HEK293-derived immunogen)

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Product Details

Catalogue Number: 500-P154HGBT
Biotinylated Anti-Human IL-12 (HEK293-derived immunogen)


Source: Polyclonal Goat

Preparation: Produced from sera of goats immunized with highly pure Recombinant Human IL-12. Anti-Human IL-12-specific antibody was purified by affinity chromatography and then biotinylated.

Immunogen: HEK293 cells-derived Recombinant Human IL-12 p70 (PeproTech catalog# 200-12H)

Sandwich ELISA: To detect Human IL-12 by sandwich ELISA (using 100ul/well) a concentration of 0.25-1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti-Human IL-12 (500-P154HG) as a capture antibody, allows the detection of at least 2000-4000 pg/ml of Recombinant Human IL-12.

Biotinylated Anti-Human IL-12 Sandwich ELISA

Western Blot: To detect Human IL-12 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible development reagents the detection limit for Recombinant Human IL-12 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Biotinylated Anti-Human IL-12 Western Blot Reduced Biotinylated Anti-Human IL-12 Western Blot Unreduced


1mg will be provided as 2x500μg.

Country Of Origin: USA

Not for human use.

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