Animal-Free Recombinant Human IL-21 0 ReviewsSubmit a Review Product Details Catalog Number: AF-200-21 Description: IL-21 is a pleiotropic cytokine produced by CD4+ T cells in response to antigenic stimulation. Its action generally enhances antigen-specific responses of immune cells. The biological effects of IL-21 include: inducing the differentiation of T cell-stimulated B cells into plasma cells and memory B cells; the stimulation of IgG production in conjunction with IL-4; and the induction of apoptotic effects in naïve B cells and stimulated B cells in the absence of T cell signaling. Additionally, IL-21 promotes the anti-tumor activity of CD8+ T-cells and NK cells. IL-21 exerts its effect through binding to a specific type I cytokine receptor, IL-21R, which also contains the γ chain (γc) found in other cytokine receptors, including IL-2, IL-4, IL-7, IL-9 and IL-15. The IL-21/IL-21R interaction triggers a cascade of events, which includes activation of the tyrosine kinases JAK1 and JAK3, followed by activation of the transcription factors STAT1 and STAT3. Recombinant Human IL-21 is a 15.4 kDa protein consisting of 132 amino acid residues. Manufactured using all Animal-Free reagents. Source: E.coli Synonyms: Interleukin-21, Za11 AA Sequence: MQDRHMIRMR QLIDIVDQLK NYVNDLVPEF LPAPEDVETN CEWSAFSCFQ KAQLKSANTG NNERIINVSI KKLKRKPPST NAGRRQKHRL TCPSCDSYEK KPPKEFLERF KSLLQKMIHQ HLSSRTHGSE DS Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses. Biological Activity: Determined by its ability to stimulate the proliferation of human ANBL-6 cells. The expected ED50 is ≤ 0.5 ng/ml, corresponding to a specific activity of ≥ 2 x106 units/mg. Calculated Molecular Weight: 15.4 kDa Accession Number: Q9HBE4 Gene ID: 59067 Endotoxin: Endotoxin level is < 0.01 ng/ug of protein (< 0.1 EU/ug) Cross Reactivity Cited in References: Human, Mouse References PubMed SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest CAR T Cell Apoptosis Cancer Immune System Stem Cells & Differentiation Recent Citations First Author Weng, R R Title Epigenetic modulation of immune synaptic-cytoskeletal networks potentiates γδ T cell-mediated cytotoxicity in lung cancer Citation Nature Communications; 12(1) pg2163 PubMed ID 33846331 First Author Gonzalez-Figueroa, P Title Follicular regulatory T cells produce neuritin to regulate B cells. Citation Cell; 184(7) pg1775-1789.e19 PubMed ID 33711260 First Author Schwerd, T Title A variant in IL6ST with a selective IL-11 signaling defect in human and mouse Citation Bone Research; 8 pg24 PubMed ID 32566365