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Recombinant Murine Noggin

Product Details

Catalogue Number: 250-38
Description:

Noggin belongs to a group of diffusible proteins that bind to ligands of the TGF-β family, and regulate their activity by inhibiting their access to signaling receptors.  Noggin was originally identified as a BMP-4 antagonist whose action was critical for proper formation of the head and other dorsal structures.  Consequently, noggin has been shown to modulate the activities of other BMPs including BMP-2,-7,-13, and -14.  Targeted deletion of noggin in mice results in prenatal death, and a recessive phenotype displaying a severely malformed skeletal system.  Conversely, transgenic mice over-expressing noggin in mature osteoblasts display impaired osteoblastic differentiation, reduced bone formation, and severe osteoporosis.  Recombinant Murine Noggin is a 46.4 kDa disulfide-linked homodimer consisting of two 206 amino acid polypeptide chains.

Source: E.coli

Synonyms: None

AA Sequence (monomer): MQHYLHIRPA PSDNLPLVDL IEHPDPIFDP KEKDLNETLL RSLLGGHYDP GFMATSPPED RPGGGGGPAG GAEDLAELDQ LLRQRPSGAM PSEIKGLEFS EGLAQGKKQR LSKKLRRKLQ MWLWSQTFCP VLYAWNDLGS RFWPRYVKVG SCFSKRSCSV PEGMVCKPSK SVHLTVLRWR CQRRGGQRCG WIPIQYPIIS ECKCSC

Purity: ≥ 95% by SDS-PAGE gel and HPLC analyses.

Biological Activity: Determined by its ability to inhibit 5.0 ng/ml of BMP-4 induced alkaline phosphatase production by ATDC5 chondrogenic cells. The expected ED50 for this effect is 1.0-2.0 ng/ml of Noggin.

Calculated Molecular Weight: 46.4 kDa

Accession Number: P97466

Gene ID: 18121

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Drurey, C
Title
Intestinal epithelial tuft cell induction is negated by a murine helminth and its secreted products.
Citation
The Journal of Experimental Medicine; 219(1)
PubMed Id
First Author
Shanahan, M T
Title
Multiomic analysis defines the first microRNA atlas across all small intestinal epithelial lineages and reveals novel markers of almost all major cell types.
Citation
American Journal of Physiology. Gastrointestinal and Liver Physiology; 321(6) pgG668-G681
PubMed Id
First Author
Sufi, J
Title
Multiplexed single-cell analysis of organoid signaling networks
Citation
Nature Protocols; 16(10) pg4897-4918
PubMed Id

product.subtitle.productreviews

Existing reviews
organoids
Works perfect with both human and murine pancreatic organoids.
From:  Mitesh | Date:  09.07.2020
Murine Noggin for CRC and pan Organoid Culture
Murine Noggin I am using for Human as well as mouse Pancreas, Colon and Rectal Organoid culture medias.
Consistent activity each lot and next day delivery.
From:  Hardik | Date:  14.08.2020
mNoggin for organoids culture
We have used it for both mouse and human pancreatic organoids and it works perfectly!
From:  Pilar | Date:  27.08.2020