B- and T-lymphocyte attenuator (BTLA), or CD272, is a type I transmembrane co-inhibitory receptor of the CD28 receptor family that is involved in modulating adaptive immune responses and T cell co-stimulation. BTLA acts in a manner similar to PD-1 and CTLA-4, the other inhibitory receptors of the CD28 family, as an anti-inflammatory molecule crucial to dampening immune response. Expressed most prominently in T cells, including Th1, Th2, CD4+, and CD8+ cells, BTLA can also be found in B cells, NK cells, dendritic cells and macrophages. Ligation of BTLA with HVEM, a member of the TNF receptor super family (TNFRSF), induces tyrosine phosphorylation of ITIM and ITSM motifs found within the BTLA cytoplasmic domain, resulting in association with SHP-1 and SHP-2. While HVEM’s interactions with the ligands LIGHT and Lymphotoxin-α induce a powerful stimulatory immune response, the interaction of BTLA and HVEM attenuates T cell proliferation and the production of cytokines including IL-2, IL-10 and IFN-γ. This interaction between BTLA and HVEM is the only receptor-ligand interaction known to bridge the CD28 and TNFR families. Recent studies provide evidence that engagement of BTLA’s third cytoplasmic motif, Grb2, may transmit a positive signal, suggesting both a co-inhibitory and co-stimulatory role for BTLA. Dysfunction of the BTLA pathway plays a role in the pathogenesis of numerous autoimmune diseases and cancers. The naturally occurring human BTLA monomer consists of a 127-amino-acid extracellular domain, a 21-amino-acid transmembrane domain, and a 111-amino-acid cytoplasmic domain. PeproTech’s CHO cell-derived Recombinant Human BTLA Fc is a glycosylated, disulfide-linked homodimer of 353-amino-acid-residues whose monomer consists of a 120-amino-acid portion of the extracellular domain fused to the 231-amino-acid length Fc portion of human IgG by two glycine residues. The calculated molecular weight of monomeric CHO cell-derived Recombinant Human BTLA Fc is 39.8 kDa; however, due to glycosylation, it migrates at an apparent molecular weight of approximately 50-55 kDa by SDS-PAGE analysis under reducing conditions.
B- and T-lymphocyte attenuator, B- and T-lymphocyte-associated protein, CD272
AA Sequence (monomer):
KESCDVQLYI KRQSEHSILA GDPFELECPV KYCANRPHVT WCKLNGTTCV KLEDRQTSWK EEKNISFFIL HFEPVLPNDN GSYRCSANFQ SNLIESHSTT LYVTDVKSAS ERPSKDEMAS GGPKSCDKTH TCPPCPAPEL LGGPSVFLFP PKPKDTLMIS RTPEVTCVVV DVSHEDPEVK FNWYVDGVEV HNAKTKPREE QYNSTYRVVS VLTVLHQDWL NGKEYKCKVS NKALPAPIEK TISKAKGQPR EPQVYTLPPS RDELTKNQVS LTCLVKGFYP SDIAVEWESN GQPENNYKTT PPVLDSDGSF FLYSKLTVDK SRWQQGNVFS CSVMHEALHN HYTQKSLSLS PGK
Calculated Molecular Weight:
≥ 95% by SDS-PAGE gel and HPLC analyses.
Determined by its ability to bind recombinant Murine HVEM Fc in a functional ELISA.