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Recombinant Human/Murine/Rat BDNF

Product Details

Catalogue Number: 450-02
Recombinant Human/Murine/Rat BDNF

BDNF is a member of the NGF family of neurotrophic growth factors. Like other members of this family, BDNF supports neuron proliferation and survival. BDNF can bind to a low affinity cell surface receptor called LNGFR, which also binds other neurotrophins such as NGF, NT-3 and NT-4. However, BDNF mediates its neurotrophic properties by signaling through a high affinity cell surface receptor called gp145/trkB. BDNF is expressed as the C-terminal portion of a 247 amino acid polypeptide precursor, which also contains a signal sequence of 18 amino acid residues and a propeptide of 110 amino acid residues. Recombinant Human/Murine/Rat BDNF is a 27.0 kDa homodimer of two 120 amino acid subunits linked by strong non-covalent interactions.

Source: E.coli

Synonyms: Brain Derived Neurotrophic Factor, Abrineurin


Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.

Biological Activity: Determined by its ability to bind recombinant human TrkB Fc Chimera in a functional ELISA.

This product has been tested against the WHO standard. International Unit information can be found here.

Recombinant Human/Murine/Rat BDNF Biological Activity Recombinant Human/Murine/Rat BDNF WHO Competitor Graph Recombinant Human/Murine/Rat BDNF Biological Comparison Graph

Calculated Molecular Weight: 27 kDa

Accession Number: P23560

Gene ID: 627

Endotoxin: Endotoxin level is < 0.1 ng/ug of protein (< 1 EU/ug)

Country Of Origin: USA

Not for human use.

Research Interest


First Author
Pantazis, C B
A reference human induced pluripotent stem cell line for large-scale collaborative studies.
Cell Stem Cell; 29(12) pg1685-1702.e22
PubMed Id
First Author
Kaluscha, S
Evidence that direct inhibition of transcription factor binding is the prevailing mode of gene and repeat repression by DNA methylation.
Nature Genetics; 54(12) pg1895-1906
PubMed Id
First Author
Mesci, P
SARS-CoV-2 infects human brain organoids causing cell death and loss of synapses that can be rescued by treatment with Sofosbuvir.
PLoS Biology; 20(11) pge3001845
PubMed Id


Existing reviews
I have used PeproTech BDNF since 2012
I have used PeproTech BDNF at Stanford and Genentech since 2012 and it has been great for differentiating stem cells into neurons. It is the best product in this category of recombinant proteins.
From:  Louise | Date:  21/08/2020
Supports Growth of Cholinergic Neurons
Routinely use this to support and maintain primary murine cholinergic neurons in culture. Excellent product.
From:  Bernadeta | Date:  26/08/2020
Differentiation and support of SH-SY5Y cells
I have been using this product for over 3 years now to support and differentiate SH-SY5Y cells in culture. It is an excellent product that many people in my lab use. It always works well and is reliable.
From:  Crystal | Date:  29/08/2020
Works great!
Used this product for intravitreal injections in the mouse eye and it worked as anticipated. Very pleased and will continue to purchase for future experiments.
From:  Rebecca | Date:  03/08/2021
Supports Growth of Motor Neurons
I use this product for supporting the maturation of MNs in vitro, it works well.
From:  meimei | Date:  04/08/2021
Cortical Neuron Differentiation
Very consistent for stem cell culturing
From:  Danielle | Date:  24/08/2021
Great recombinant protein
This is a very good recombinant protein to elicit TrkB signaling in cultured neurons at 50 ng/ml concentration or support differentiation of mouse ESC-s to neurons. We haven’t experienced lot to lot variation and delivery is always prompt. We highly recommend.
From:  Epp | Date:  31/08/2021