Facteurs de Croissance et Cytokines

Recombinant Murine sRANK Ligand (E.coli derived)

Product Details

Catalogue Number: 315-11
Description:

RANKL and RANK are members of the TNF superfamily of ligands and receptors that play an important role in the regulation of specific immunity and bone turnover. RANK (receptor) was originally identified as a dendritic cell-membrane protein, which, by interacting with RANKL, augments the ability of dendritic cells. These dendritic cells then stimulate naïve T-cell proliferation in a mixed lymphocyte reaction, promote the survival of RANK+ T-cells, and regulate T-cell-dependent immune response. RANKL, which is expressed in a variety of cells, including osteoblasts, fibroblasts, activated T-cells and bone marrow stromal cells, is also capable of interacting with a decoy receptor called OPG. Binding of soluble OPG to sRANKL inhibits osteoclastogenesis by interrupting the signaling between stromal cells and osteoclastic progenitor cells, thereby leading to excess accumulation of bone and cartilage. Recombinant Murine sRANKL is a 19.4 kDa polypeptide comprising the TNF-homologous region of RANKL (174 amino acid residues).

Source: E.coli

Synonyms: soluble Receptor Activator of NF-κB Ligand, TNFSF11, TRANCE (TNF-related activation-induced cytokine), OPGL, ODF (Osteoclast differentiation factor)

AA Sequence: PAMMEGSWLD VAQRGKPEAQ PFAHLTINAA SIPSGSHKVT LSSWYHDRGW AKISNMTLSN GKLRVNQDGF YYLYANICFR HHETSGSVPT DYLQLMVYVV KTSIKIPSSH NLMKGGSTKN WSGNSEFHFY SINVGGFFKL RAGEEISIQV SNPSLLDPDQ DATYFGAFKV QDID

Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.

Biological Activity: Determined by its dose-dependent ability to induce reporter gene in HT-29 NF-κB Luc reporter cells.

Calculated Molecular Weight: 19.4 kDa

Accession Number: O35235

Gene ID: 21943

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Ma, J
Title
TRPV6 is a potential regulator of bone resorption in bone loss induced by estrogen deficiency.
Citation
Iscience; 24(11) pg103261
PubMed Id
First Author
He, Y
Title
Serglycin induces osteoclastogenesis and promotes tumor growth in giant cell tumor of bone
Citation
Cell Death & Disease; 12(10) pg868
PubMed Id
First Author
Ambrosi, T H
Title
Aged skeletal stem cells generate an inflammatory degenerative niche
Citation
Nature; 597(7875) pg256-262
PubMed Id

product.subtitle.productreviews

Existing reviews
RANKL for Osteoclast Induction
Works very well!  Along with m-csf, makes mighty fine osteoclasts!  Is true to expiration as stated in TDS as well.
From:  Amy | Date:  26/08/2020