Recombinant Murine sRANK Ligand (E.coli derived) 1 ReviewsSubmit a Review Product Details Catalogue Number: 315-11 Description: RANKL and RANK are members of the TNF superfamily of ligands and receptors that play an important role in the regulation of specific immunity and bone turnover. RANK (receptor) was originally identified as a dendritic cell-membrane protein, which, by interacting with RANKL, augments the ability of dendritic cells. These dendritic cells then stimulate naïve T-cell proliferation in a mixed lymphocyte reaction, promote the survival of RANK+ T-cells, and regulate T-cell-dependent immune response. RANKL, which is expressed in a variety of cells, including osteoblasts, fibroblasts, activated T-cells and bone marrow stromal cells, is also capable of interacting with a decoy receptor called OPG. Binding of soluble OPG to sRANKL inhibits osteoclastogenesis by interrupting the signaling between stromal cells and osteoclastic progenitor cells, thereby leading to excess accumulation of bone and cartilage. Recombinant Murine sRANKL is a 19.4 kDa polypeptide comprising the TNF-homologous region of RANKL (174 amino acid residues). Source: E.coli Synonyms: soluble Receptor Activator of NF-κB Ligand, TNFSF11, TRANCE (TNF-related activation-induced cytokine), OPGL, ODF (Osteoclast differentiation factor) AA Sequence: PAMMEGSWLD VAQRGKPEAQ PFAHLTINAA SIPSGSHKVT LSSWYHDRGW AKISNMTLSN GKLRVNQDGF YYLYANICFR HHETSGSVPT DYLQLMVYVV KTSIKIPSSH NLMKGGSTKN WSGNSEFHFY SINVGGFFKL RAGEEISIQV SNPSLLDPDQ DATYFGAFKV QDID Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses. Biological Activity: Determined by its dose-dependent ability to induce reporter gene in HT-29 NF-κB Luc reporter cells. Calculated Molecular Weight: 19.4 kDa Accession Number: O35235 Gene ID: 21943 crossreactivity: Cow + Mouse, Human, Mouse, Rat References PubMed SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest Apoptosis Bone, Skeletal, Cartilage Cancer Diabetes/Weight Regulation Immune System TNF Superfamily product.subtitle.recentcitations First Author Katsuyama, E Title Interleukin-1 receptor-associated kinase-4 (IRAK4) promotes inflammatory osteolysis by activating osteoclasts and inhibiting formation of foreign body giant cells. Citation The Journal of Biological Chemistry; 290(2) pg716-26 PubMed Id 25404736 First Author Faict, S Title Exosomes play a role in multiple myeloma bone disease and tumor development by targeting osteoclasts and osteoblasts. Citation Blood Cancer Journal; 8(11) pg105 PubMed Id 30409995 First Author Rocha, F R Title Relevance of Caspase-1 and Nlrp3 Inflammasome on Inflammatory Bone Resorption in A Murine Model of Periodontitis. Citation Scientific Reports; 10(1) pg7823 PubMed Id 32385413 product.subtitle.productreviews Existing reviews RANKL for Osteoclast Induction Works very well! Along with m-csf, makes mighty fine osteoclasts! Is true to expiration as stated in TDS as well. From: Amy | Date: 26/08/2020 × RANKL for Osteoclast Induction Works very well! Along with m-csf, makes mighty fine osteoclasts! Is true to expiration as stated in TDS as well. From: Amy | Date: 26/08/2020 Close