Antigen Affinity Purified Polyclonal Antibodies

Anti-Human TNF-α (Polyclonal Rabbit)

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Product Details

Catalogue Number: 500-P31A
Description:
Anti-Human TNF-α (Polyclonal Rabbit)
 

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure recombinant Human TNF-α. Anti-Human TNF-α specific antibody was purified by affinity chromatography employing immobilized Human TNF-α matrix.

Immunogen: E.coli derived Recombinant Human TNF-α (PeproTech catalog# 300-01A)

Sandwich ELISA: To detect Human TNF-α by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human TNF-α (500-P31ABt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human TNF-α.

Anti-Human TNF-α Sandwich ELISA

Western Blot: To detect Human TNF-α by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human TNF-α is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Human TNF-α Western Blot Reduced Anti-Human TNF-α Western Blot Unreduced

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Ma, L
Title
A novel small-molecule tumor necrosis factor α inhibitor attenuates inflammation in a hepatitis mouse model.
Citation
The Journal of Biological Chemistry; 289(18) pg12457-66
PubMed Id
First Author
Green, J A
Title
Mycobacterium tuberculosis-infected human monocytes down-regulate microglial MMP-2 secretion in CNS tuberculosis via TNFα, NFκB, p38 and caspase 8 dependent pathways.
Citation
Journal of Neuroinflammation; 8 pg46
PubMed Id
First Author
Cohen-Or, I
Title
AGEs secreted by bacteria are involved in the inflammatory response.
Citation
PLoS ONE; 6(3) pge17974
PubMed Id