Anti-Human TNF-α (Monoclonal Mouse) 0 ReviewsSubmit a Review Product Details Catalogue Number: 500-M26 Source: Monoclonal Mouse Preparation: Produced in BALB/c mice immunized with highly pure Recombinant Human TNF-α. Anti-Human TNF- α-specific antibody was purified from cell culture by Protein G affinity chromatography. Immunogen: E.coli derived Recombinant Human TNF-α (PeproTech catalog# 300-01A) Sandwich ELISA: In a sandwich ELISA (assuming 100μl/well), a concentration of 2.0-4.0 μg/ml of this antibody will detect at least 1000 pg/ml of Recombinant Human TNF-α when used with PeproTech’s Biotinylated Antigen Affinity Purified Anti-Human TNF-α (500-P31ABT) as the detection antibody at a concentration of at least 0.5-1.0 μg/ml. Western Blot: To detect Human TNF-α by Western Blot analysis this antibody can be used at a concentration of 0.5-1.0 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human TNF-α is 0.5-1.0 ng/lane, under reducing conditions and 0.25-0.5 ng/lane, under non-reducing conditions. Note: Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information. crossreactivity: Human, Mouse References SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest COVID-19 AIDS/HIV Angiogenesis/Cardiovascular Apoptosis Cancer Diabetes/Weight Regulation Immune System Inflammation Neurobiology Stem Cells & Differentiation TNF Superfamily Allergy Transplantation product.subtitle.recentcitations First Author Tselepis, C Title Tumour necrosis factor-alpha in Barrett's oesophagus: a potential novel mechanism of action. Citation Oncogene; 21(39) pg6071-81 PubMed Id 12203119 First Author Miller, J L Title The type I NADH dehydrogenase of Mycobacterium tuberculosis counters phagosomal NOX2 activity to inhibit TNF-alpha-mediated host cell apoptosis. Citation PLoS Pathogens; 6(4) pge1000864 PubMed Id 20421951 First Author Vitale, M Title NK-dependent DC maturation is mediated by TNFalpha and IFNgamma released upon engagement of the NKp30 triggering receptor. Citation Blood; 106(2) pg566-71 PubMed Id 15784725