Animal-Free Recombinant Human Heregulinβ-1 0 ReviewsSubmit a Review Product Details Catalog Number: AF-100-03 Description: Neuregulin/Heregulin is a family of structurally-related polypeptide growth factors derived from alternatively spliced genes (NRG1, NRG2, NRG3 and NRG4). To date, there are over 14 soluble and transmembrane proteins derived from the NRG1 gene. Proteolytic processing of the extracellular domain of the transmembrane NRG1 isoforms releases soluble growth factors. HRG1-β1 contains an Ig domain and an EGF-like domain; the latter is necessary for direct binding to receptor tyrosine kinases erb3 and erb4. This binding induces erb3 and erb4 heterodimerization with erb2, stimulating intrinsic kinase activity that leads to tyrosine phosphorylation. Although HRG1-β1's biological effects are still unclear, it has been found to promote motility and invasiveness of breast cancer cells, which may also involve up-regulation of expression and function of the autocrine motility-promoting factor (AMF). Recombinant Human Heregulinβ-1 (HRG1-B1) is a 7.5 kDa polypeptide consisting of only the EGF domain of heregulinβ-1 (65 amino acid residues). Manufactured using all Animal-Free reagents. Source: E.coli Synonyms: Neuregulin1 (NRG1-β1), Neu differentiation factor (rat), HRG, HRG1-β1, Breast cancer cell differentiation factor p45, ARIA (Acetylcholine Receptor Inducting Activity), glial growth factor AA Sequence: SHLVKCAEKE KTFCVNGGEC FMVKDLSNPS RYLCKCPNEF TGDRCQNYVM ASFYKHLGIE FMEAE Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses. Biological Activity: The ED50 was determined by the dose-dependent stimulation of the proliferation of human MCF-7 cells is ≤ 0.5 ng/ml, corresponding to a specific activity of ≥ 2 x 106 units/mg. Calculated Molecular Weight: 7.5 kDa Accession Number: Q02297 Gene ID: 3084 Endotoxin: Endotoxin level is < 0.01 ng/ug of protein (< 0.1 EU/ug) Cross Reactivity Cited in References: Human, Mouse, Rat References PubMed SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest Organoids Angiogenesis/Cardiovascular Apoptosis Cancer Immune System Neurobiology Stem Cells & Differentiation Recent Citations First Author Jacques, M Title CODEX, a neural network approach to explore signaling dynamics landscapes. Citation Molecular Systems Biology; 17(4) pge10026 PubMed ID 33835701 First Author Cui, Y Title The NF2 tumor suppressor merlin interacts with Ras and RasGAP, which may modulate Ras signaling. Citation Oncogene; 38(36) pg6370-6381 PubMed ID 31312020 First Author Patel, A Title Dystrophin deficiency leads to dysfunctional glutamate clearance in iPSC derived astrocytes. Citation Translational Psychiatry; 9(1) pg200 PubMed ID 31434868