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Growth Factors & Cytokines

Recombinant Human TNF-α

제품 상세

카다로그 번호 300-01A
제품 설명

TNF-α is a pleiotropic pro-inflammatory cytokine secreted by various cells, including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts. It belongs to the TNF family of ligands, and signals through two receptors, TNFR1 and TNFR2. TNF-α is cytotoxic to a wide variety of tumor cells, and is an essential factor in mediating the immune response against bacterial infections. TNF-α also plays a role in the induction of septic shock, autoimmune diseases, rheumatoid arthritis, inflammation, and diabetes. Human and murine TNF-α demonstrate significant cross-species reactivity. TNF-α exists in two forms; a type II transmembrane protein, and a mature soluble protein. The TNF-α transmembrane protein is proteolitically cleaved to yield a soluble, biologically active, 17 kDa TNF-α, which forms a non-covalently linked homotrimer in solution. Recombinant Human TNF-α is a soluble 157 amino acid protein (17.4 kDa) which corresponds to C-terminal extracellular domain of the full length transmembrane protein.

Source: E.coli

Synonyms: Tumor Necrosis Factor, TNFSF2, Cachectin, Differentiation-inducing factor (DIF), Necrosin, Cytotoxin

AA Sequence: VRSSSRTPSD KPVAHVVANP QAEGQLQWLN RRANALLANG VELRDNQLVV PSEGLYLIYS QVLFKGQGCP STHVLLTHTI SRIAVSYQTK VNLLSAIKSP CQRETPEGAE AKPWYEPIYL GGVFQLEKGD RLSAEINRPD YLDFAESGQV YFGIIAL

Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.

Biological Activity: The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 107 units/mg.

Calculated Molecular Weight: 17.4 kDa

Accession Number: P01375

Gene ID: 7124

crossreactivity: Research Interest

Country Of Origin: USA

Not for human use.

product.subtitle.productreviews

기존 리뷰
Great recombinant protein from peprotech
We use this recombinant TNFa in one of our assays and it has performed extremely well/consistent over the years. I would definitely recommend this product to people who are interested.
에서:  Di | 날짜:  2020-07-08
Reliable TNF both for human and mouse
Using this item for over 12 years.
Always gives consistent results.
Note- very sensitive to freezing and thawing.
We keep it in 100ug/ml, 10ul aliquotes for use and minimize repeated freeze/thaw of the vial.
Works well at 10 ng/ml - 100 ng/ml to study inflammatory gene expression.
에서:  Param | 날짜:  2020-07-09
in vitro probiotic properties assay
We routinely use recombinant human TNF-α in co-incubation with HT29 cells to characterization in vitro of anti-inflammatory probiotic properties of potential candidate strains used IL-8 as readout. This product as Prepothec indicates is sensitive to thawed cycles, I recommend prepare aliquots (100ng/ul).
에서:  Edgar | 날짜:  2020-07-26