Growth Factors & Cytokines

Recombinant Human TNF-α

Product Details

Catalogue Number: 300-01A
Description:

TNF-α is a pleiotropic pro-inflammatory cytokine secreted by various cells, including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts. It belongs to the TNF family of ligands, and signals through two receptors, TNFR1 and TNFR2. TNF-α is cytotoxic to a wide variety of tumor cells, and is an essential factor in mediating the immune response against bacterial infections. TNF-α also plays a role in the induction of septic shock, autoimmune diseases, rheumatoid arthritis, inflammation, and diabetes. Human and murine TNF-α demonstrate significant cross-species reactivity. TNF-α exists in two forms; a type II transmembrane protein, and a mature soluble protein. The TNF-α transmembrane protein is proteolitically cleaved to yield a soluble, biologically active, 17 kDa TNF-α, which forms a non-covalently linked homotrimer in solution. Recombinant Human TNF-α is a soluble 157 amino acid protein (17.4 kDa) which corresponds to C-terminal extracellular domain of the full length transmembrane protein.

Source: E.coli

Synonyms: Tumor Necrosis Factor, TNFSF2, Cachectin, Differentiation-inducing factor (DIF), Necrosin, Cytotoxin

AA Sequence: VRSSSRTPSD KPVAHVVANP QAEGQLQWLN RRANALLANG VELRDNQLVV PSEGLYLIYS QVLFKGQGCP STHVLLTHTI SRIAVSYQTK VNLLSAIKSP CQRETPEGAE AKPWYEPIYL GGVFQLEKGD RLSAEINRPD YLDFAESGQV YFGIIAL

Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.

Biological Activity: The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 107 units/mg.

This product has been tested against the WHO standard. International Unit information can be found here.

Calculated Molecular Weight: 17.4 kDa

Accession Number: P01375

Gene ID: 7124

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Johnstone, R W
Title
P-glycoprotein protects leukemia cells against caspase-dependent, but not caspase-independent, cell death.
Citation
Blood; 93(3) pg1075-85
PubMed Id
First Author
Mócsai, A
Title
Adhesion-dependent degranulation of neutrophils requires the Src family kinases Fgr and Hck.
Citation
Journal of immunology (Baltimore, Md. : 1950); 162(2) pg1120-6
PubMed Id
First Author
Gupta, S
Title
Signaling pathways mediated by the TNF- and cytokine-receptor families target a common cis-element of the IFN regulatory factor 1 promoter.
Citation
Journal of immunology (Baltimore, Md. : 1950); 161(11) pg5997-6004
PubMed Id

product.subtitle.productreviews

Existing reviews
Great recombinant protein from peprotech
We use this recombinant TNFa in one of our assays and it has performed extremely well/consistent over the years. I would definitely recommend this product to people who are interested.
From:  Di | Date:  7/8/2020
Reliable TNF both for human and mouse
Using this item for over 12 years.
Always gives consistent results.
Note- very sensitive to freezing and thawing.
We keep it in 100ug/ml, 10ul aliquotes for use and minimize repeated freeze/thaw of the vial.
Works well at 10 ng/ml - 100 ng/ml to study inflammatory gene expression.
From:  Param | Date:  7/9/2020
in vitro probiotic properties assay
We routinely use recombinant human TNF-α in co-incubation with HT29 cells to characterization in vitro of anti-inflammatory probiotic properties of potential candidate strains used IL-8 as readout. This product as Prepothec indicates is sensitive to thawed cycles, I recommend prepare aliquots (100ng/ul).
From:  Edgar | Date:  7/26/2020
Easy to reconstitute and store for long-term usage
This is useful in some of our inflammation and fibrosis studies. It has a decent shelf like in -20C of few months and gets used well before then. Recommendation is to make aliquots of 50-100 ul of 10 ug/ml stocks.
From:  Vikram | Date:  8/3/2021