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Recombinant Human TNF-α

Product Details

Catalogue Number: 300-01A
Description:
Recombinant Human TNF-α

TNF-α is a pleiotropic pro-inflammatory cytokine secreted by various cells, including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts. It belongs to the TNF family of ligands, and signals through two receptors, TNFR1 and TNFR2. TNF-α is cytotoxic to a wide variety of tumor cells, and is an essential factor in mediating the immune response against bacterial infections. TNF-α also plays a role in the induction of septic shock, autoimmune diseases, rheumatoid arthritis, inflammation, and diabetes. Human and murine TNF-α demonstrate significant cross-species reactivity. TNF-α exists in two forms; a type II transmembrane protein, and a mature soluble protein. The TNF-α transmembrane protein is proteolitically cleaved to yield a soluble, biologically active, 17 kDa TNF-α, which forms a non-covalently linked homotrimer in solution. Recombinant Human TNF-α is a soluble 157 amino acid protein (17.4 kDa) which corresponds to C-terminal extracellular domain of the full length transmembrane protein.

Source: E.coli

Synonyms: Tumor Necrosis Factor, TNFSF2, Cachectin, Differentiation-inducing factor (DIF), Necrosin, Cytotoxin

AA Sequence: VRSSSRTPSD KPVAHVVANP QAEGQLQWLN RRANALLANG VELRDNQLVV PSEGLYLIYS QVLFKGQGCP STHVLLTHTI SRIAVSYQTK VNLLSAIKSP CQRETPEGAE AKPWYEPIYL GGVFQLEKGD RLSAEINRPD YLDFAESGQV YFGIIAL

Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.

Biological Activity: The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 107 units/mg.

This product has been tested against the WHO standard. International Unit information can be found here.

Recombinant Human TNF-α WHO Comparison Graph Recombinant Human TNF-α Biological Activity Graph

Calculated Molecular Weight: 17.4 kDa

Accession Number: P01375

Gene ID: 7124

Endotoxin: Endotoxin level is < 0.1 ng/ug of protein (< 1 EU/ug)

crossreactivity:
Bacteria, Cow, Dog, Human, Human + Hamster, Human + Mouse, Human + Rat, Human + Virus, Monkey, Mouse, Pig, Rat, Sheep
Country Of Origin: USA

Not for human use.

Research Interest
COVID-19 AIDS/HIV Angiogenesis/Cardiovascular Apoptosis Cancer Diabetes/Weight Regulation Immune System Inflammation Neurobiology Stem Cells & Differentiation TNF Superfamily Allergy Transplantation

product.subtitle.recentcitations

First Author
Klaus, F
Title
PI3Kγ is a novel regulator of TNFα signaling in the human colon cell line HT29/B6.
Citation
Annals of the New York Academy of Sciences; 1515(1) pg196-207
PubMed Id
35725890
First Author
Loef, E J
Title
Plasminogen and plasmin can bind to human T cells and generate truncated CCL21 that increases dendritic cell chemotactic responses.
Citation
The Journal of Biological Chemistry; 298(7) pg102112
PubMed Id
35690148
First Author
Guo, Z
Title
Durable complete response to neoantigen-loaded dendritic-cell vaccine following anti-PD-1 therapy in metastatic gastric cancer.
Citation
NPJ Precision Oncology; 6(1) pg34
PubMed Id
35661819

product.subtitle.productreviews

Existing reviews
Great recombinant protein from peprotech
We use this recombinant TNFa in one of our assays and it has performed extremely well/consistent over the years. I would definitely recommend this product to people who are interested.
From:  Di | Date:  08/07/2020
Reliable TNF both for human and mouse
Using this item for over 12 years.
Always gives consistent results.
Note- very sensitive to freezing and thawing.
We keep it in 100ug/ml, 10ul aliquotes for use and minimize repeated freeze/thaw of the vial.
Works well at 10 ng/ml - 100 ng/ml to study inflammatory gene expression.
From:  Param | Date:  09/07/2020
in vitro probiotic properties assay
We routinely use recombinant human TNF-α in co-incubation with HT29 cells to characterization in vitro of anti-inflammatory probiotic properties of potential candidate strains used IL-8 as readout. This product as Prepothec indicates is sensitive to thawed cycles, I recommend prepare aliquots (100ng/ul).
From:  Edgar | Date:  26/07/2020
Easy to reconstitute and store for long-term usage
This is useful in some of our inflammation and fibrosis studies. It has a decent shelf like in -20C of few months and gets used well before then. Recommendation is to make aliquots of 50-100 ul of 10 ug/ml stocks.
From:  Vikram | Date:  03/08/2021