Growth Factors & Cytokines

Recombinant Human IFN-β

Product Details

Catalog Number: 300-02BC
Description:

Proteins of this family play an important role in inducing non-specific resistance against a broad range of viral infections.  They also affect cell proliferation and modulate immune responses.  Produced by peripheral blood leukocytes and lymphoblastoid cells, IFN-α is an acid-stable molecule that signals through IFN-α/βR, which is also used by IFN-β.  Both IFNs have similar anti-viral activity and regulate expression of MHC class I antigens.  IFN-α contains four highly conserved cysteine residues that form two disulfide bonds, one of which is necessary for biological activity.  PeproTech’s Recombinant Human IFN-β is a 20.0 kDa protein containing 166 amino acid residues. Due to glycosylation, IFN-β has an approximate MW of 22.3 kDa based on SDS-PAGE gel and Mass Spectrometry.

*Human IFN-β (Catalog Number 300-02BC) has replaced Human IFN-β (Catalog Number 300-02B)

Source: CHO cells

Synonyms: Fibroblast Interferon, IFNB1, Type I Interferon

AA Sequence: MSYNLLGFLQ RSSNFQCQKL LWQLNGRLEY CLKDRMNFDI PEEIKQLQQF QKEDAALTIY EMLQNIFAIF RQDSSSTGWN ETIVENLLAN VYHQINHLKT VLEEKLEKED FTRGKLMSSL HLKRYYGRIL HYLKAKEYSH CAWTIVRVEI LRNFYFINRL TGYLRN

Purity: ≥ 95% by SDS-PAGE gel and HPLC analyses.

Biological Activity: Assay #1: Measured by its ability to induce apoptosis in HeLa cells. The expected ED50 for this effect is 20-30 ng/ml.

Assay #2: Determined by a cytotoxicity assay using human TF-1 cells. The expected ED50 is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg.

Note:

1mg will be provided as 2x500μg.

Cross Reactivity:
Human, Monkey, Mouse
Research Interest

Country Of Origin: USA

Research Use Only: Not for use in diagnostic or therapeutic procedures.