Growth Factors & Cytokines

Recombinant Human IL-34

Product Details

Catalogue Number: 200-34
Description:

IL-34 is a homodimeric cytokine that is expressed in a range of tissues that include spleen, heart, brain, liver, kidney, lung, ovary, thymus, testis, small intestine, prostate and colon. IL-34 is a ligand for colony-stimulating factor-1 receptor (CSF1R), which also binds to CSF-1. It specifically binds CD14+ monocytes, promotes survival and proliferation of human peripheral blood monocytes, and stimulates macrophage colony formation by human bone marrow cells. IL-34, like human CSF-1, stimulates phosphorylation of MAPK1/ERK2 and MAPK3/ERK1. Recombinant Human IL-34 is a 52.5 kDa homodimeric glycoprotein consisting of 460 amino acid residues, including a C-terminal His-Tag.

Source: HEK293 cells

Synonyms: Interleukin-34, C16orf77

AA Sequence (monomer): NEPLEMWPLT QNEECTVTGF LRDKLQYRSR LQYMKHYFPI NYKISVPYEG VFRIANVTRL QRAQVSEREL RYLWVLVSLS ATESVQDVLL EGHPSWKYLQ EVETLLLNVQ QGLTDVEVSP KVESVLSLLN APGPNLKLVR PKALLDNCFR VMELLYCSCC KQSSVLNWQD CEVPSPQSCS PEPSLQYAAT QLYPPPPWSP SSPPHSTGSV RPVRAQGEGL LPHHHHHHHH

Purity: ≥ 95% by SDS-PAGE gel and HPLC analyses.

Biological Activity: Measured by its ability to induce secretion of MCP-1 from PBMCs.

Calculated Molecular Weight: 52.5 kDa

Accession Number: Q6ZMJ4

Gene ID: 146433

Note:

1mg will be provided as 2x500μg.

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Nzou, G
Title
Human Cortex Spheroid with a Functional Blood Brain Barrier for High-Throughput Neurotoxicity Screening and Disease Modeling.
Citation
Scientific Reports; 8(1) pg7413
PubMed Id
First Author
Konttinen, H
Title
PSEN1ΔE9, APPswe, and APOE4 Confer Disparate Phenotypes in Human iPSC-Derived Microglia.
Citation
Stem Cell Reports; 13(4) pg669-683
PubMed Id
First Author
Liu, T
Title
Multi-omic comparison of Alzheimer's variants in human ESC-derived microglia reveals convergence at APOE.
Citation
The Journal of Experimental Medicine; 217(12)
PubMed Id

product.subtitle.productreviews

Existing reviews
IL-34 for microglia differentiation
Our lab has been successfully using IL-34 to differentiate stem cells into microglia for over 1.5 year. This product goes through a rigorous quality control and always gets delivered in a timely manner.
From:  Morgane | Date:  7/21/2020