Growth Factors & Cytokines

Animal-Free Recombinant Human EGF

Product Details

Catalogue Number: AF-100-15

EGF is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. Additionally, EGF has been shown to inhibit gastric secretion, and to be involved in wound healing. EGF signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor. This receptor also binds with TGF-α and VGF (vaccinia virus growth factor). Recombinant Human EGF is a 6.2 kDa globular protein containing 53 amino acid residues, including 3 intramolecular disulfide bonds.

*Animal-Free Recombinant Human EGF (Catalog Number AF-100-15) has replaced Recombinant Human EGF (Catalog Number 100-15)

Manufactured using all Animal-Free reagents.

Source: E.coli

Synonyms: Epidermal Growth Factor, Urogastrone, URG


Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.

Biological Activity: The ED50 was determined by a cell proliferation assay using balb/c 3T3 cells is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg.

This product has been tested against the WHO standard. International Unit information can be found here.

Calculated Molecular Weight: 6.2 kDa

Accession Number: P01133

Gene ID: 1950

Country Of Origin: USA

Not for human use.

Research Interest


First Author
Tang, T
Protease Nexin I is a feedback regulator of EGF/PKC/MAPK/EGR1 signaling in breast cancer cells metastasis and stemness.
Cell Death & Disease; 10(9) pg649
PubMed Id
First Author
Sommer, U
Mechanisms of SOCS3 phosphorylation upon interleukin-6 stimulation. Contributions of Src- and receptor-tyrosine kinases.
The Journal of Biological Chemistry; 280(36) pg31478-88
PubMed Id
First Author
Sparta, B
Receptor Level Mechanisms Are Required for Epidermal Growth Factor (EGF)-stimulated Extracellular Signal-regulated Kinase (ERK) Activity Pulses.
The Journal of Biological Chemistry; 290(41) pg24784-92
PubMed Id


Existing reviews
Quality EGF for Organoid Culture
one of best quality growth factors from peprotech, we are using for various organoid media. Consistent quality, very very cost effective than competitor suppliers, next day delivery.
From:  Hardik | Date:  14/08/2020
Constant quality for in vitro culture
Using for in vitro culture. Constant quality. Also quick delivery.
From:  KOHSUKE | Date:  24/08/2021
EGF for primary cell culture
I have used Peprotech human recombinant EGF and FGF for the culture of primary glioblastoma cells for several years now. Works well, no complaints and any questions I have had have been answered quickly and thoroughly by Peprotech customer service
From:  Kathleen | Date:  24/08/2021
EGF, human basic recombinant, E. coli
Using the EGF for 5 years now in our culture medium and are very satisfied ! Critical cells, but they grow very well with the EGF (and FGF) of Peprotech !
From:  Arjan | Date:  27/08/2021
Great recombinant protein
Works very nicely in combination with FGF to keep mouse ESC-derived NPCs alive and proliferating in culture (at 10 ng/ml concentration). Highly recommend.
From:  Epp | Date:  31/08/2021