Biotinylated Anti-Human Galectin-1 0 ReviewsSubmit a Review Product Details Catalog Number: 500-P210BT Description: Source: Polyclonal Rabbit Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hGalectin-1. Anti-Human Galectin-1 specific antibody was purified by affinity chromatography and then biotinylated. Immunogen: E.coli derived Recombinant Human Galectin-1 (PeproTech catalog# 450-39) Sandwich ELISA: To detect hGalectin-1 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti-Human Galectin-1 (500-P210) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hGalectin-1. Western Blot: To detect hGalectin-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hGalectin-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Note: 1mg will be provided as 2x500μg. Cross Reactivity Cited in References: Human References SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest Apoptosis Cancer Immune System Recent Citations First Author Kopcow, H D Title T cell apoptosis at the maternal-fetal interface in early human pregnancy, involvement of galectin-1. Citation Proceedings of the National Academy of Sciences of the United States of America; 105(47) pg18472-7 PubMed ID 19011096