Growth Factors & Cytokines

Recombinant Murine Adiponectin

Product Details

Catalogue Number: 315-26
Description:

Adiponectin is an adipose-derived secreted protein containing 236 amino acid residues.  It is relatively abundant in humans and rodents, accounting for about 0.01% of total plasma protein.  The circulating levels of adiponectin are decreased under conditions of obesity, insulin resistance, and type II diabetes.  Disruption of adiponectin in mice causes insulin resistance and neointimal formation.  Conversely, administration of recombinant adiponectin suppresses hepatic glucose production, and reverses insulin resistance associated with both lipoatrophy and obesity.  The protective role of adiponectin is attributed to its anti-inflammatory properties (e.g. ability to suppress expression of TNF-α and class A scavenger receptor in macrophages). Recombinant Murine Adiponectin is a multimeric glycoprotein, which has a calculated molecular weight of 25.8 kDa and contains amino acids Val-21 to Asn-247 of the adiponectin precursor protein fused to an N-terminal histidine tag. As a result of glycosylation, Recombinant Murine Adiponectin migrates with an apparent molecular mass of approximately 31-36 kDa by SDS-PAGE gel, under reducing conditions.

Source: Hi-5 Insect cells

Synonyms: Acrp-30, GBP-28, APM-1

AA Sequence: RGHHHHHHHH VTTTEELAPA LVPPPKGTCA GWMAGIPGHP GHNGTPGRDG RDGTPGEKGE KGDAGLLGPK GETGDVGMTG AEGPRGFPGT PGRKGEPGEA AYVYRSAFSV GLETRVTVPN VPIRFTKIFY NQQNHYDGST GKFYCNIPGL YYFSYHITVY MKDVKVSLFK KDKAVLFTYD QYQEKNVDQA SGSVLLHLEV GDQVWLQVYG DGDHNGLYAD NVNDSTFTGF LLYHDTN

Purity: ≥ 95% by SDS-PAGE gel and HPLC analyses.

Biological Activity: Determined by a cytotoxic assay using M1 cells.  The ED50 for this effect is 4.0-6.0 μg/ml.

Calculated Molecular Weight: 25.8 kDa

Accession Number: Q60994

Gene ID: 11450

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Takenaga, K
Title
Obesity reduces the anticancer effect of AdipoRon against orthotopic pancreatic cancer in diet-induced obese mice.
Citation
Scientific Reports; 11(1) pg2923
PubMed Id
First Author
Grases-Pintó, B
Title
Enhancement of immune maturation in suckling rats by leptin and adiponectin supplementation.
Citation
Scientific Reports; 9(1) pg1786
PubMed Id
First Author
Liu, Z
Title
Adiponectin reduces ER stress-induced apoptosis through PPARα transcriptional regulation of ATF2 in mouse adipose.
Citation
Cell death & disease; 7(11) pge2487
PubMed Id