Monoclonal Antibodies

Anti-Human IL-8 (CXCL8) (Monoclonal Mouse)

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Product Details

Catalogue Number: 500-M08
Description:


Anti-Human IL-8 (CXCL8) (Monoclonal Mouse)

Preparation: Produced in mice immunized with highly pure Recombinant Human IL-8 (CXCL8). Anti-Human IL-8 (CXCL8)-specific antibody was purified from cell culture by Protein G affinity chromatography.

Source: Monoclonal Mouse

Immunogen: E.coli derived Recombinant Human IL-8 (CXCL8) (72 a.a.) (PeproTech catalog# 200-08M)

Sandwich ELISA: In a sandwich ELISA (assuming 100μl/well), a concentration of 2.0-4.0 μg/ml of this antibody will detect at least 1000pg/ml of Recombinant human IL-8 when used with PeproTech’s Biotinylated Antigen Affinity Purified Anti-Human IL-8 (CXCL8) (500-P28BT) as the detection antibody at a concentration of at least 0.5- 1.0 μg/ml.

Anti-Human IL-8 (CXCL8) Sandwich ELISA

Western Blot: To detect Human IL-8 by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human IL-8 is 2.0-4.0 ng/lane, under non-reducing conditions.

Anti-Human IL-8 (CXCL8) Western Blot Unreduced

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Maertzdorf, J
Title
IL-17 expression in human herpetic stromal keratitis: modulatory effects on chemokine production by corneal fibroblasts.
Citation
Journal of immunology (Baltimore, Md. : 1950); 169(10) pg5897-903
PubMed Id
First Author
Cassatella, M A
Title
Toll-like receptor-3-activated human mesenchymal stromal cells significantly prolong the survival and function of neutrophils.
Citation
Stem cells (Dayton, Ohio); 29(6) pg1001-11
PubMed Id
First Author
Fudala, R
Title
Anti-IL-8 autoantibody:IL-8 immune complexes suppress spontaneous apoptosis of neutrophils.
Citation
American Journal of Physiology. Lung Cellular and Molecular Physiology; 293(2) pgL364-74
PubMed Id