Antigen Affinity Purified Polyclonal Antibodies

Anti-Rat GRO-β/MIP-2 (CXCL2)

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Product Details

Catalogue Number: 500-P75

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure recombinant Rat GROβ/MIP-2. Anti-Rat GROβ/MIP-2 specific antibody was purified by affinity chromatography employing immobilized Rat GROβ/MIP-2 matrix.

Immunogen: E.coli derived Recombinant Rat GRO-β/MIP-2 (CXCL2) (PeproTech catalog# 400-11)

Sandwich ELISA: To detect Rat GROβ/MIP-2 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Rat GROβ/MIP-2 (500-P75Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat GROβ/MIP-2.

Anti-Rat GRO-β/MIP-2 (CXCL2) Sandwich ELISA

Western Blot: To detect Rat GROβ/MIP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Rat GROβ/MIP-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Rat GRO-β/MIP-2 (CXCL2) Western Blot Reduced Anti-Rat GRO-β/MIP-2 (CXCL2) Western Blot Unreduced


Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

Country Of Origin: USA

Not for human use.

Research Interest


First Author
Kuwata, H
Search of factors that intermediate cytokine-induced group IIA phospholipase A2 expression through the cytosolic phospholipase A2- and 12/15-lipoxygenase-dependent pathway.
The Journal of Biological Chemistry; 280(27) pg25830-9
PubMed Id
First Author
Nabah, Y N
Angiotensin II induces neutrophil accumulation in vivo through generation and release of CXC chemokines.
Circulation; 110(23) pg3581-6
PubMed Id
First Author
Mateo, T
A critical role for TNFalpha in the selective attachment of mononuclear leukocytes to angiotensin-II-stimulated arterioles.
Blood; 110(6) pg1895-902
PubMed Id