Growth Factors & Cytokines

Recombinant Human IGF-II

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Product Details

Catalogue Number: 100-12
Description:

The IGFs are mitogenic, polypeptide growth factors that stimulate the proliferation and survival of various cell types, including muscle, bone, and cartilage tissue in vitro.  IGFs are predominantly produced by the liver, although a variety of tissues produce the IGFs at distinctive times.  The IGFs belong to the Insulin gene family, which also contains insulin and relaxin.  The IGFs are similar to insulin by structure and function, but have a much higher growth-promoting activity than insulin.  IGF-II expression is influenced by placenta lactogen, while IGF-I expression is regulated by growth hormone.  Both IGF-I and IGF-II signal through the tyrosine kinase type I receptor (IGF-IR), but IGF-II can also signal through the IGF-II/Mannose-6-phosphate receptor.  Mature IGFs are generated by proteolytic processing of inactive precursor proteins, which contain N-terminal and C-terminal propeptide regions.  Recombinant Human IGF-I and IGF-II are globular proteins containing 70 and 67 amino acids, respectively, and 3 intra-molecular disulfide bonds.  The calculated molecular weight of Recombinant Human IGF-II is 7.5 kDa.

Source: E.coli

Synonyms: Insulin-like Growth Factor-II, Somatamedin A

AA Sequence: AYRPSETLCG GELVDTLQFV CGDRGFYFSR PASRVSRRSR GIVEECCFRS CDLALLETYC ATPAKSE

Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.

Biological Activity: The ED50 was determined by a cell proliferation assay using FDC-P1 cells is ≤ 2.0 ng/ml, corresponding to a specific activity of ≥ 5 x 105 units/mg.

This product has been tested against the WHO standard. International Unit information can be found here.

Calculated Molecular Weight: 7.5 kDa

Accession Number: P01344

Gene ID: 3481

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Uenishi, G I
Title
NOTCH signaling specifies arterial-type definitive hemogenic endothelium from human pluripotent stem cells.
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Nature Communications; 9(1) pg1828
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Savchenko, A
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Growth factors regulate phototransduction in retinal rods by modulating cyclic nucleotide-gated channels through dephosphorylation of a specific tyrosine residue.
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Proceedings of the National Academy of Sciences of the United States of America; 98(10) pg5880-5
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Zhou, X
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Circuit Design Features of a Stable Two-Cell System.
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Cell; 172(4) pg744-757.e17
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