Recombinant Human M-CSF

Product Details

Catalogue Number: 300-25

Description:

M-CSF is a potent hematopoietic factor produced by a variety of cells, including lymphocytes, monocytes, fibroblasts, endothelial cells, myoblasts and osteoblasts. It is a key regulator of cellular proliferation, differentiation, and survival for blood monocytes, tissue macrophages, and their respective progenitor cells. M-CSF has been shown to play important roles in modulating dermal thickness and fertility. M-CSF is clinically used in the treatment of infection, malignancies and atherosclerosis. It facilitates hematopoietic recovery after bone marrow transplantation. Human M-CSF is reactive in murine systems, but the murine molecule exhibits no activity on human cells. Recombinant Human M-CSF is a 36.8 kDa homodimeric protein consisting of two 159 amino acid polypeptide subunits.

Source: E.coli

Synonyms: Macrophage Colony Stimulating Factor, CSF-1, MGI-IM

AA Sequence (monomer): MEEVSEYCSH MIGSGHLQSL QRLIDSQMET SCQITFEFVD QEQLKDPVCY LKKAFLLVQD IMEDTMRFRD NTPNAIAIVQ LQELSLRLKS CFTKDYEEHD KACVRTFYET PLQLLEKVKN VFNETKNLLD KDWNIFSKNC NNSFAECSSQ GHERQSEGS

Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.

Biological Activity: The ED₅₀ was determined by the dose-dependent stimulation of the proliferation of murine M-NSF-60 cells ≤ 1 ng/ml, corresponding to a specific activity of ≥ 1 x 10⁶ units/mg.

This product has been tested against the WHO standard. International Unit information can be found here.

Calculated Molecular Weight: 36.8 kDa

Accession Number: P09603

Gene ID: 1435

Endotoxin: Endotoxin level is < 0.1 ng/ug of protein (< 1 EU/ug)

crossreactivity:

Human, Human + Cow, Human + Hamster, Human + Virus, Monkey, Mouse, Rabbit, Rat

Country Of Origin: USA

Not for human use.

Research Interest

Cancer

Immune System

Stem Cells & Differentiation

product.subtitle.recentcitations

First Author

George, S

Title

Protocol to drive human monocyte-to-macrophage polarization in vitro using tumor conditioned media.

Citation

STAR Protocols; 3(4) pg101666

PubMed Id

36125932

First Author

Di Carlo, S

Title

GM-CSF suppresses antioxidant signaling and drives IL-1β secretion through NRF2 downregulation.

Citation

EMBO reports; 23(8) pge54226

PubMed Id

35695080

First Author

Park, J

Title

Multi-Omics-Based Autophagy-Related Untypical Subtypes in Patients with Cerebral Amyloid Pathology.

Citation

Advanced science (Weinheim, Baden-Wurttemberg, Germany); 9(23) pge2201212

PubMed Id

35694866

product.subtitle.productreviews

Existing reviews

Great Product

It is a great product for my study. I had used this product in several cell-line experiments including primary cell lines.

From: Kanagavel | Date: 22/07/2020

Perfect for macrophages

M-CSF is wonderful for macrophage tissue culture.

From: Heather | Date: 03/08/2021

Best MCSF on the market

My lab switched to Peprotech a few years ago for human MCSF and we will never go back. We generate human macrophages with this product and have only had consistent and great results. It's also competively priced for the quantity and so I'd definitely recommend it.

From: Emilio | Date: 04/08/2021

Growth Factors & Cytokines