Growth Factors & Cytokines

Recombinant Human sFas Ligand

0 ReviewsSubmit a Review

Product Details

Catalogue Number: 310-03H
Description:


Recombinant Human sFas Ligand

Fas Ligand (FasL) is a member of the TNF superfamily that is expressed on the cell surface of activated T cells. Binding of FasL to Fas Receptor triggers apoptosis in Fas-bearing cells. FasL has the ability to kill T cells and activated B cells, which leads to down-regulation of the immune response. The mechanism of Fas-induced apoptosis involves recruitment of pro-caspase 8 through an adaptor molecule called FADD, followed by processing of the pro-enzyme into active forms. These active caspases then cleave various cellular substrates, leading to the eventual cell death. Both human and murine sFasL are fully active on human and murine cells. Recombinant Human soluble Fas Ligand is a 17.9 kDa protein comprising the TNF-homologous region of FasL and contains an 8-residue N-terminal His-Tag.

Source: CHO cells

Synonyms: soluble Fas Ligand (sFasL), TNFSF6, CD95L, Apo I Ligand, APTL

AA Sequence: HHHHHHHHPS PPPEKKELRK VAHLTGKSNS RSMPLEWEDT YGIVLLSGVK YKKGGLVINE TGLYFVYSKV YFRGQSCNNL PLSHKVYMRN SKYPQDLVMM EGKMMSYCTT GQMWARSSYL GAVFNLTSAD HLYVNVSELS LVNFEESQTF FGLYKL

Purity: ≥ 95% by SDS-PAGE gel and HPLC analyses.

Biological Activity: Determined by its ability to induce cytotoxicity in Jurkat cells in the absence of any cross-linking. The ED50 for this effect is ≤ 10.0 ng/ml, corresponding to a specific activity of ≥ 1 x 105 units/mg.

Calculated Molecular Weight: 17.9 kDa

Accession Number: P48023

Gene ID: 356

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Lin, W
Title
Helicobacter pylori sensitizes TNF-related apoptosis-inducing ligand (TRAIL)-mediated apoptosis in human gastric epithelial cells through regulation of FLIP.
Citation
Cell death & disease; 5 pge1109
PubMed Id
First Author
Valiente, M
Title
Serpins promote cancer cell survival and vascular co-option in brain metastasis.
Citation
Cell; 156(5) pg1002-16
PubMed Id
First Author
Zhao, Q
Title
MUC1 extracellular domain confers resistance of epithelial cancer cells to anoikis.
Citation
Cell death & disease; 5 pge1438
PubMed Id