Growth Factors & Cytokines

Recombinant Human/Murine/Rat Activin A (Insect derived)

Product Details

Catalogue Number: 120-14
Description:
Recombinant Human/Murine/Rat Activin A (Insect derived)

Activin A is a TGF-β family member that exhibits a wide range of biological activities, including regulation of cellular proliferation and differentiation, and promotion of neuronal survival.  Elevated levels of Activin A in human colorectal tumors and in postmenopausal women have been implicated in colorectal and breast cancers, respectively.  The biological activities of Activin A can be neutralized by inhibins and by the diffusible TGF-β antagonist, follistatin.  Activin A binds to the two forms of activin receptor type I (Act RI-A and Act RI-B) and two forms of activin receptor type II (Act RII-A and Act RII-B).  Activins are homodimers or heterodimers of different β subunits.  They are produced as precursor proteins with an amino terminal propeptide that is cleaved to release the C-terminal bioactive ligand.  Recombinant Human/Murine/Rat Activin A is a 26.0 kDa disulfide-linked homodimer of two βA chains, each containing 116 amino acid residues.

Source: Hi-5 Insect cells

Synonyms: Inhibin beta-1, FRP, FSH (Follicle-Stimulating Hormone)-releasing protein, Erythroid Differentiation Factor (EDF)

AA Sequence (monomer): GLECDGKVNI CCKKQFFVSF KDIGWNDWII APSGYHANYC EGECPSHIAG TSGSSLSFHS TVINHYRMRG HSPFANLKSC CVPTKLRPMS MLYYDDGQNI IKKDIQNMIV EECGCS

Purity: ≥ 95% by SDS-PAGE gel and HPLC analyses.

Biological Activity: Determined by its ability to inhibit the proliferation of mouse MPC-11 cells. The expected ED50 is ≤ 2.0 ng/ml, corresponding to a specific activity of ≥ 5 x 105 units/mg.

This product has been tested against the WHO standard. International Unit information can be found here.

Calculated Molecular Weight: 26 kDa

Accession Number: P08476

Gene ID: 3624

Note:

1mg will be provided as 2x500μg.

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Huang, X
Title
OCT4 cooperates with distinct ATP-dependent chromatin remodelers in naïve and primed pluripotent states in human.
Citation
Nature Communications; 12(1) pg5123
PubMed Id
First Author
Karabicici, M
Title
Doxorubicin-induced senescence promotes stemness and tumorigenicity in EpCAM-/CD133- nonstem cell population in hepatocellular carcinoma cell line, HuH-7.
Citation
Molecular Oncology; 15(8) pg2185-2202
PubMed Id
First Author
Scheibner, K
Title
Epithelial cell plasticity drives endoderm formation during gastrulation
Citation
Nature Cell Biology; 23(7) pg692-703
PubMed Id

product.subtitle.productreviews

Existing reviews
A superior product
My lab has been using this product for many years and it always works perfectly. We once purchased it from a different manufacturer due to a relative lower price. However, we found that we must increase the dose if the Activin A is not from Peprotech. As such, the cost went up as we had to use higher dose to make it work. Hence, we learnt that Activin A’s purity and quality from Peprotech is super... reviews.showmore
From:  Sha | Date:  08/07/2020
Excellent Product
I have been using the recombinant Activin A for 5 years for the culture of chicken primordial germ cells (cPGCs) and have had excellent results. PeproTech has been great to work with as well.
From:  Jeffery | Date:  26/08/2020