Growth Factors & Cytokines

Recombinant Human/Murine/Rat Activin A (CHO derived)

Product Details

Catalogue Number: 120-14P
Description:

Activin A is a TGF-β family member that exhibits a wide range of biological activities, including regulation of cellular proliferation and differentiation, and promotion of neuronal survival. Elevated levels of Activin A in human colorectal tumors and in postmenopausal women have been implicated in colorectal and breast cancers, respectively. The biological activities of Activin A can be neutralized by inhibins and by the diffusible TGF-β antagonist, follistatin. Activin A binds to the two forms of activin receptor type I (Act RI-A and Act RI-B) and two forms of activin receptor type II (Act RII-A and Act RII-B). Activins are homodimers or heterodimers of different β subunits. They are produced as precursor proteins with an amino terminal propeptide that is cleaved to release the C-terminal bioactive ligand. Recombinant Human/Murine/Rat Activin A is a 26.0 kDa disulfide-linked homodimer of two βA chains, each containing 116 amino acid residues.

Source: CHO cells

Synonyms: Inhibin beta-1, FRP, FSH (Follicle-Stimulating Hormone)-releasing protein, Erythroid Differentiation Factor (EDF)

AA Sequence (monomer): GLECDGKVNI CCKKQFFVSF KDIGWNDWII APSGYHANYC EGECPSHIAG TSGSSLSFHS TVINHYRMRG HSPFANLKSC CVPTKLRPMS MLYYDDGQNI IKKDIQNMIV EECGCS

Purity: ≥ 95% by SDS-PAGE gel and HPLC analyses.

Biological Activity: Determined by its ability to inhibit the proliferation of mouse MPC-11 cells. The expected ED50 is ≤ 2.0 ng/ml, corresponding to a specific activity of ≥ 5 x 105 units/mg.

This product has been tested against the WHO standard. International Unit information can be found here.

Calculated Molecular Weight: 26 kDa

Accession Number: P08476

Gene ID: 3624

Note:

1mg will be provided as 2x500μg.

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Yang, J
Title
GATA6-AS1 Regulates GATA6 Expression to Modulate Human Endoderm Differentiation.
Citation
Stem Cell Reports; 15(3) pg694-705
PubMed Id
First Author
Lau, B
Title
The Expression of Human Cytomegalovirus MicroRNA MiR-UL148D during Latent Infection in Primary Myeloid Cells Inhibits Activin A-triggered Secretion of IL-6.
Citation
Scientific Reports; 6 pg31205
PubMed Id
First Author
Diekmann, U
Title
Chemically defined and xenogeneic-free differentiation of human pluripotent stem cells into definitive endoderm in 3D culture.
Citation
Scientific Reports; 9(1) pg996
PubMed Id

product.subtitle.productreviews

Existing reviews
Great for definitive endoderm--reliable and reproducible!
I use this activin A for the definitive endoderm stages of my human iPSC differentiations. This component of their media is pretty critical, and I have had reproducible success getting cells through this phase using Peprotech. Other labs differentiating stem cells have had similar success. This product will be a repeat order for me!
From:  Kendall | Date:  26/08/2020