Animal-Free Recombinant Human TGF-β1 (CHO derived) 0 ReviewsSubmit a Review Product Details Catalogue Number: AF-100-21C Description: The three mammalian isoforms of TGF-β, TGF-β1, β2, and β3, signal through the same receptor and elicit similar biological responses. They are multifunctional cytokines that regulate cell proliferation, growth, differentiation and motility, as well as synthesis and deposition of the extracellular matrix. They are involved in various physiological processes, including embryogenesis, tissue remodeling and wound healing. They are secreted predominantly as latent complexes, which are stored at the cell surface and in the extracellular matrix. The release of the biologically active TGF-β isoform from a latent complex involves proteolytic processing of the complex and/or induction of conformational changes by proteins such as thrombospondin-1. TGF-b1 is the most abundant isoform secreted by almost every cell type. It was originally identified for its ability to induce phenotypic transformation of fibroblasts, and recently it has been implicated in the formation of skin tumors. Recombinant Human TGF-β1 is a 25.0 kDa protein composed of two identical 112 amino acid polypeptide chains linked by a single disulfide bond. Manufactured using all Animal-Free reagents. Source: CHO cells Synonyms: Transforming Growth Factor-β1, Differentiation inhibiting factor, Cartilage-inducing factor AA Sequence (monomer): ALDTNYCFSS TEKNCCVRQL YIDFRKDLGW KWIHEPKGYH ANFCLGPCPY IWSLDTQYSK VLALYNQHNP GASAAPCCVP QALEPLPIVY YVGRKPKVEQ LSNMIVRSCK CS Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses. Biological Activity: Determined by TGF-β1’s ability to inhibit the mouse IL-4-dependent proliferation of mouse HT-2 cells. The expected ED50 is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 107 units/mg. This product has been tested against the WHO standard. International Unit information can be found here. Calculated Molecular Weight: 25 kDa Accession Number: P01137 Gene ID: 7040 Note: 1mg will be provided as 2x500μg. crossreactivity: Human, Mouse, Pig References PubMed SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest Angiogenesis/Cardiovascular Apoptosis Bone, Skeletal, Cartilage Cancer Cell Culture Immune System Inflammation Neurobiology Stem Cells & Differentiation TGF-β Superfamily Wound Healing Allergy product.subtitle.recentcitations First Author Grauel, A L Title TGFβ-blockade uncovers stromal plasticity in tumors by revealing the existence of a subset of interferon-licensed fibroblasts. Citation Nature Communications; 11(1) pg6315 PubMed Id 33298926 First Author Yamashita, T Title An orally-active adiponectin receptor agonist mitigates cutaneous fibrosis, inflammation and microvascular pathology in a murine model of systemic sclerosis. Citation Scientific Reports; 8(1) pg11843 PubMed Id 30087356 First Author Breuls, N Title Valproic acid stimulates myogenesis in pluripotent stem cell-derived mesodermal progenitors in a NOTCH-dependent manner Citation Cell Death & Disease; 12(7) pg677 PubMed Id 34226515