Animal-Free Recombinant Human TGF-β1 (CHO derived) 0 ReviewsSubmit a Review Product Details Catalogue Number: AF-100-21C Description: The three mammalian isoforms of TGF-β, TGF-β1, β2, and β3, signal through the same receptor and elicit similar biological responses. They are multifunctional cytokines that regulate cell proliferation, growth, differentiation and motility, as well as synthesis and deposition of the extracellular matrix. They are involved in various physiological processes, including embryogenesis, tissue remodeling and wound healing. They are secreted predominantly as latent complexes, which are stored at the cell surface and in the extracellular matrix. The release of the biologically active TGF-β isoform from a latent complex involves proteolytic processing of the complex and/or induction of conformational changes by proteins such as thrombospondin-1. TGF-b1 is the most abundant isoform secreted by almost every cell type. It was originally identified for its ability to induce phenotypic transformation of fibroblasts, and recently it has been implicated in the formation of skin tumors. Recombinant Human TGF-β1 is a 25.0 kDa protein composed of two identical 112 amino acid polypeptide chains linked by a single disulfide bond. Manufactured using all Animal-Free reagents. Source: CHO cells Synonyms: Transforming Growth Factor-β1, Differentiation inhibiting factor, Cartilage-inducing factor AA Sequence (monomer): ALDTNYCFSS TEKNCCVRQL YIDFRKDLGW KWIHEPKGYH ANFCLGPCPY IWSLDTQYSK VLALYNQHNP GASAAPCCVP QALEPLPIVY YVGRKPKVEQ LSNMIVRSCK CS Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses. Biological Activity: Determined by TGF-β1’s ability to inhibit the mouse IL-4-dependent proliferation of mouse HT-2 cells. The expected ED50 is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 107 units/mg. This product has been tested against the WHO standard. International Unit information can be found here. Calculated Molecular Weight: 25 kDa Accession Number: P01137 Gene ID: 7040 Endotoxin: Endotoxin level is < 0.01 ng/ug of protein (< 0.1 EU/ug) Note: 1mg will be provided as 2x500μg. crossreactivity: Human, Mouse, Pig References PubMed SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest Angiogenesis/Cardiovascular Apoptosis Bone, Skeletal, Cartilage Cancer Cell Culture Immune System Inflammation Neurobiology Stem Cells & Differentiation TGF-β Superfamily Wound Healing Allergy product.subtitle.recentcitations First Author Liu, W Title hUC-MSCs Attenuate Acute Graft-Versus-Host Disease through Chi3l1 Repression of Th17 Differentiation. Citation Stem Cells International; 2022 pg1052166 PubMed Id 36277038 First Author Dole, N S Title Mechanosensitive miR-100 coordinates TGFβ and Wnt signaling in osteocytes during fluid shear stress. Citation FASEB journal : official publication of the Federation of American Societies for Experimental Biology; 35(10) pge21883 PubMed Id 34569659 First Author Özcan, A Title Programmable RNA targeting with the single-protein CRISPR effector Cas7-11 Citation Nature; 597(7878) pg720-725 PubMed Id 34489594